rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: RNA methodologies
    sub title: laboratory guide for isolation and characterization
  name:
    name part: Farrell, Robert E.
    name part: Jr
    role:
      role term: creator
  name:
    name part: ScienceDirect (Online service)
  type of resource: text
  genre: bibliography
  genre: Electronic books.
  genre: Laboratory Manual
  genre: Laboratory manuals
  genre: Laboratory manuals.
  genre: Manuels de laboratoire.
  origin info:
    place:
      place term: ne
    place:
      place term: Amsterdam
    place:
      place term: Boston
    publisher: Elsevier/Academic Press
    date issued: 2010
    edition: 4th ed.
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource (xxiii, 717 pages) : illustrations
  abstract: This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary.
  table of contents: CHAPTER 1: RNA and the Cellular Biochemistry Revisited -- CHAPTER 2: RNA Isolation Strategies -- CHAPTER 3: The Truth about Tissues -- CHAPTER 4: Going Green: RNA and the Molecular Biology of Plants (NEW) -- CHAPTER 5: Isolation of Polyadenylated RNA -- CHAPTER 6: Quality Control for RNA Preparations (EXPANDED) -- CHAPTER 7: Resilient Ribonucleases -- CHAPTER 8: Stringency: Conditions that Influence Nucleic Acid Structure (EXPANDED) -- CHAPTER 9: Electrophoresis of RNA (EXPANDED) -- CHAPTER 10: Photodocumentation and Image Analysis (EXPANDED) -- CHAPTER 11: Northern Analysis -- CHAPTER 12: Nucleic Acid Probe Technology -- CHAPTER 13: Practical Nucleic Acid Hybridization -- CHAPTER 14: Principles of Detection (EXPANDED) -- CHAPTER 15: Quantification of Specific mRNAs by Nuclease Protection -- CHAPTER 16: Analysis of Nuclear RNA (EXPANDED) -- CHAPTER 17: cDNA Synthesis (EXPANDED) -- CHAPTER 18: RT-PCR (NEW) -- CHAPTER 19: Quantitative PCR Techniques -- CHAPTER 20: Functional Genomics and Transcript Profiling (NEW) -- CHAPTER 21: High-Throughput Analysis of Gene Expression -- CHAPTER 22: Non-Array Methods for Global Analysis of Gene Expression (NEW) -- CHAPTER 23: RNA Interference: Targeted Gene Silencing (EXPANDED) -- CHAPTER 24: Genomes, Transcriptomes, Proteomes, and Bioinformatics -- CHAPTER 25: An RNA Paradigm.
  note: Robert E. Farrell Jr.
  note: Previous edition: 2005.
  note: Includes bibliographical references and index.
  note: English.
  note: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force. WlAbNL
  subject:
    topic: RNA
    topic: Analysis
    genre: Laboratory manuals
  subject:
    topic: RNA
    topic: isolation & purification
  subject:
    topic: Molecular Biology
    topic: methods
  subject:
    topic: RNA
    topic: physiology
  subject:
    topic: ARN
    topic: Analyse
    genre: Manuels de laboratoire
  subject:
    topic: SCIENCE
    topic: Life Sciences
    topic: Molecular Biology
  subject:
    topic: RNA
    topic: Analysis
  subject:
    topic: rna
  subject:
    topic: laboratoriummethoden
  subject:
    topic: laboratory methods
  subject:
    topic: methodologie
  subject:
    topic: methodology
  subject:
    topic: moleculaire biologie
  subject:
    topic: molecular biology
  subject:
    topic: moleculaire genetica
  subject:
    topic: molecular genetics
  subject:
    topic: protocollen
  subject:
    topic: protocols
  subject:
    topic: Molecular Genetics
  subject:
    topic: Moleculaire genetica
  classification: QP623 .F37 2010
  classification: 572.88
  classification: 2009 K-772
  classification: QU 25 F245r 2010
  related item:
    title info:
      title: RNA methodologies
    name:
      name part: Farrell, Robert E.
    origin info:
      publisher: Amsterdam ; Boston : Elsevier/Academic Press, 2010
      edition: 4th ed.
    identifier: (DLC) 2005041012
    identifier: (OCoLC)496226598
  identifier: 9780123747273
  identifier: 0123747279
  identifier: 9780080884950
  identifier: 0080884954
  identifier: 1282329987
  identifier: 9781282329980
  identifier: 9786612329982
  identifier: 661232998X
  identifier: 166590:166792 Elsevier Science & Technology
  identifier: http://proxy.library.tamu.edu/login
  access condition: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force. WlAbNL
  record info:
    record content source: OPELS
    record creation date: 100225
    record change date: 20260326163923.4
    record identifier: in00005739399
    language of cataloging:
      language term: eng