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The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.
Outline of volumes 135-139, 141-145 : Volume 135. Immobilized enzymes and cells (Part B) -- Section I. Immobilization techniques for biocatalysts -- Section II. Immobilization techniques for cells/organelles -- Section III. Application of immobilized enzymes/cells to fundamental studies in biochemistry -- Volume 136. Immobilized enzymes and cells (Part C) -- Section I. Multistep enzyme systems and coenzymes -- Section II. Immobilized enzymes/cells in organic synthesis -- Section III. Enzyme engineering (Enzyme technology) -- Volume 137. Immobilized enzymes and cells (Part D) -- Section I. Analytical applications with emphasis on biosensors -- A. Bioselective electrodes -- B. Hybrid, cell, and tissue electrodes -- C. Bioluminescence-immobilized systems -- D. Thermistor probes -- E. Enzyme transfers -- F. Enzyme reactors -- G. Continuous monitoring -- H. Optical methods -- I. Miscellaneous new techniques -- Section II. Medical applications -- Section III. Novel techniques for and aspects of immobilized enzymes and cells -- Volume 138. Complex carbohydrates (Part E) -- Section I. Analytical methods -- Section II. Preparations -- Section III. Carbohydrate-binding proteins -- Section IV. Biosynthesis -- Section V. Degradation -- Volume 139. Cellular regulators (Part A : Calcium- and calmodulin-binding proteins) -- Section I. Isolation and characterization of calcium-binding proteins -- Section II. Molecular cloning of calcium-binding proteins, cDNAs, and genes -- Section III. Reagents and methods for the study of calcium-binding proteins -- Section IV. Regulation of calcium and calcium-binding proteins -- Volume 141. Cellular regulators (Part B : Calcium and lipids) -- Section I. Measurement and perturbation of intracellular calcium -- Section II. Inositol phospholipids and proximal metabolites -- A. Quantitation and metabolism in cells, permeable cells, and cell-free systems -- B. Regulation of lipid synthesis -- C. Guanine nucleotide-binding component -- Section III. Phorbol esters and diacylglycerols : Preparation, quantitation, and binding -- Section IV. Prostaglandins, icosanoids, leukotrienes, and other lipids -- Section V. Protein kinase C -- Section VI. New probes -- Section VII. Cross-index to prior volumes -- Volume 142. Metabolism of aromatic amino acids and amines -- Section I. Catabolism of the aromatic amino acids -- Section II. Biosynthesis of the aromatic amino acids -- Section III. Methods of determination and metabolism of the aromatic amines -- Volume 143. Sulfur and sulfur amino acids -- Section I. Separation and analysis -- A. Inorganic sulfur and selenium compounds -- B. Thiols and disulfides -- C. Other organic sulfur and selenium compounds -- Section II. Preparative methods -- A. Preparation of specific metabolites -- B. General preparative techniques -- C. Nutritional methods -- Section III. Enzymes -- A. Inorganic sulfur and sulfate -- B. Sulfur amino acids : Mammalian systems -- C. Sulfur amino acids : Plants -- D. Sulfur amino acids : Microbial systems -- E. Enzymes active with disulfides -- Volume 144. Structural and contractile proteins (Part D : Extracellular matrix) -- Biochemistry of the major components of the extracellular matrix -- A. Collagens -- B. Elastins -- C. Proteoglycans -- D. Glycoproteins -- Volume 145. Structural and contractile proteins (Part E : Extracellular matrix) -- Section I. Physical and immunohistochemical methods -- Section II. Genetic anomalies of the extracellular matrix -- Section III. Biochemistry of the extracellular matrix of specific tissues -- A. Mineralized tissues (Bone and dentin) -- B. Other tissues.
Outline of volumes 146-156 : Volume 146. Peptide growth factors (Part A) -- Section I. Epidermal growth factor -- Section II. Transforming growth factors -- Section III. Somatomedin/insulin-like growth factors -- Section IV. Bone and cartilage growth factors -- Section V. Techniques for the study of growth factor activity : Assays, phosphorylation, and surface membrane effects -- Volume 147. Peptide growth factors (Part B) -- Section I. Platelet-derived growth factor -- Section II. Angiogenesis, endothelial and fibroblast growth factors -- Section III. Nerve and glial growth factors -- Section IV. Transferrin, erythropoietin, and related factors -- Section V. Techniques for the study of growth factor activity : Genetic approaches and biological effects -- Volume 148. Plant cell membranes -- Section I. Cells, protoplasts, and liposomes -- Section II. Vacuoles and tonoplasts -- Section III. Plastids -- Section IV. Mitochondria -- Section V. Peroxisomes and glyoxysomes -- Section VI. Nuclei, endoplasmic reticulum, and plasma membrane -- Section VII. General physical and biochemical methods -- Volume 149. Drug and enzyme targeting (Part B) -- Section I. Cell targeting techniques -- Section II. Liposome carriers -- Section III. Cellular carriers -- Volume 150. Immunochemical techniques (Part K : In Vitro models of B and T cell functions and lymphoid cell receptors) -- Section I. Methods for the stimulation of lymphocytes -- Section II. In Vitro models and assays of B and T lymphocyte differentiation and function -- Section III. Receptors on lymphoid cells -- Volume 151. Molecular genetics of mammalian cells -- Section I. Cell lines useful for genetic analysis -- Section II. Special techniques for mutant selection -- Section III. Genetic mapping and analysis -- Section IV. Isolation and detection of mutant genes -- Section V. Gene regulation in tissue culture -- Volume 152. Guide to molecular cloning techniques -- Section I. Requirements for a molecular biology laboratory -- Section II. General methods for isolating and characterizing nucleic acids -- Section III. Enzymatic techniques and recombinant DNA technology -- Section IV. Restriction enzymes -- Section V. Growth and maintenance of bacteria -- Section VI. Genomic cloning -- Section VII. Preparation and characterization of RNA -- Section VIII. Preparation of cDNA and the generation of cDNA libraries -- Section IX. Selection of clones from libraries -- Section X. Identification and characterization of specific clones -- Volume 153. Recombinant DNA (Part D) -- Section I. Vectors for cloning DNA -- Section II. Vectors for expression of cloned genes -- Volume 154. Recombinant DNA (Part E) -- Section I. Methods for cloning cDNA -- Section II. Identification of cloned genes and mapping of genes -- Section III. Chemical synthesis and analysis of oligodeoxynucleotides -- Section IV. Site-specific mutagenesis and protein engineering -- Volume 155. Recombinant DNA (Part F) -- Section I. Restriction enzymes -- Section II. Rapid methods for DNA sequence analysis -- Section III. Miscellaneous methods -- Volume 156. Biomembranes (Part P : ATP-driven pumps and related transport : The NA, K-Pump) -- Section I. Preparation of Na⁺, K⁺-ATPase and subunits -- Section II. Assay of Na⁺, K⁺-ATPase activity -- Section III. Reconstitution of Na, K-Pump activity -- Section IV. Analysis of the pump cycle -- Section V. Measurement of ligand binding and distance between ligands -- Section VI. Measurements of conformational states of Na⁺, K⁺-ATPase -- Section VII. Modification of Na⁺, K⁺-ATPase -- Section VIII. Magnetic resonance studies of Na⁺, K⁺-ATPase -- Section IX. Biogenesis and membrane assembly -- Section X. Microscopy of Na⁺, K⁺-ATPase.
Outline of volumes 157-167 : Volume 157. Biomembranes (Part Q : ATP-driven pumps and related transport : Calcium, proton, and potassium pumps) -- Section I. CA²⁺ fluxes and regulation -- A. Complex systems and subcellular fractions involved in CA²⁺ regulation -- B. Characterization of CA²⁺-pumps and modulators from various sources -- C. CA²⁺ and other channels -- Section II. ATP-driven proton pumps -- Section III. ATP-driven K⁺ pumps -- Addendum -- Volume 158. Metallobiochemistry (Part A) -- Section I. Sample preparation -- Section II. Analytical techniques -- Section III. Analysis of metals -- Volume 159. Initiation and termination of cyclic nucleotide action -- Section I. Cyclic nucleotide cascades -- Section II. Assays of cyclic nucleotide levels, turnover, and transport -- Section III. Cyclic nucleotide action -- A. Cyclic nucleotide action in mammals -- B. Nonmammalian cAMP binding proteins -- C. Molecular genetic approaches -- Section IV. Protein phosphatases -- Section V. General methods for studies of phosphodiesterases -- Section VI. Methods for isolation and studies of various phosphodiesterase isoenzymes -- A. Calmodulin-stimulated phosphodiesterase -- B. cGMP-binding phosphodiesterases -- C. High-affinity cAMP phosphodiesterases -- D. Nonmammalian cyclic nucleotide phosphodiesterases -- Volume 160. Biomass (Part A : Cellulose and hemicellulose) -- Section I. Cellulose -- A. Preparation of cellulosic substrates -- B. Assays for cellulolytic enzymes -- C. Chromatographic methods for carbohydrates -- D. Miscellaneous methods for cellulolytic enzymes -- E. Purification of cellulose-degrading enzymes cellulases -- 1,4-[beta]-D-Glucan glucanohydrolases (Endocellulases) -- 1-4-[beta]-D-Glucan cellobiohydrolases (Exocellulases) -- 1,4-[beta]-D-Glucosidases -- Cellobiose dehydrogenases -- Assays of cellulolytic enzymes and miscellaneous enzymes involved in cellulolysis -- Section II. Hemicellulose -- A. Preparation of substrates for hemicellulases -- B. Analysis of [beta]-Glucan and enzyme assays -- C. Purification of hemicellulose-degrading enzymes -- Volume 161. Biomass (Part B : Lignin, pectin, and chitin) -- Section I. Lignin -- A. Preparation of substrates for ligninases -- B. Assays for ligninases -- C. Chemical methods for characterization of lignin -- D. Chromatographic methods for lignin and related compounds -- E. Nucleic acid preparations related to lignin degradation -- F. Purification of lignin-degrading enzymes -- Section II. Pectin -- A. Assays for pectin-degrading enzymes -- B. Purification of pectin-degrading enzymes -- Section III. Chitin -- A.Preparation of substrates for chitin-degrading enzymes -- B. Assay for chitin-degrading enzymes -- C. Analytical methods for chitin -- D. Purification of chitin-degrading enzymes -- Volume 162. Immunochemical techniques (Part L : Chemotaxis and inflammation) -- Section I. Chemotaxis -- A. Methods for the study of chemotaxis -- B. Methods for the study of chemoattractants and biochemistry of chemotaxis -- Section II. Inflammation -- A. Methods for the study of the cellular phenomena of inflammation and experimental models of inflammation -- B. Methods for the study of complement in chemotaxis and inflammation -- Volume 163. Immunochemical techniques (Part M : Chemotaxis and inflammation) -- Section I. Methods for the study of the biochemistry of inflammation -- Section II. Methods for the study of acute-phase reactants -- Section III. Methods for the study of repair processes in inflammation -- Volume 164. Ribosomes -- Section I. Electron microscopy -- Section II. Other biophysical methods -- Section III. Protein-RNA interactions -- Section IV. Cross-linking and affinity-labeling methods -- Section V. Chemical and enzymatic probing methods -- Section VI. Immunological methods -- Section VII. Isolation of ribosomal proteins -- Section VIII. Ribosome function and kinetics -- Section IX. Genetics -- Section X. Mathematical and computer analysis of ribosomal sequences -- Volume 165. Microbial toxins : Tools in enzymology -- Section I. Preparation of toxins -- A. Gram-positive cocci -- B. Gram-positive bacilli -- C. Gram-negative cocci -- D. Gram-negative bacilli -- E. Vibrios -- F. Immunotoxins -- Section II. Assay of toxins -- Addenda -- Volume 166. Branched-chain amino acids -- Section I. Analytical and synthetic models -- Section II. Enzyme assay -- Section III. Enzymes -- Section IV. Use of animals and animal organs in the study of branched-chain amino acid metabolism -- Volume 167. Cyanobacteria -- Section I. Isolation, identification, and culturing of cyanobacteria -- Section II. Ultrastructure, inclusions, and differentiation -- Section III. Membranes, pigments, redox reactions, and nitrogen fixation -- Section IV. Physiology and metabolism -- Section V. General physical methods -- Section VI. Molecular genetics -- Addendum.
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