RNA turnover in eukaryotes : Nucleases, pathways and analysis of mRNA decay /

Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a...

Full description

Bibliographic Details
Corporate Author: ScienceDirect (Online service)
Other Authors: Maquat, Lynne (Editor), Kiledjian, Megerditch (Editor)
Format: eBook
Language:English
Published: San Diego, California ; London : Academic Press/Elsevier, ©2008.
Series:Methods in enzymology ; v. 448.
Subjects:
Online Access:Connect to the full text of this electronic book

MARC

Tag First Indicator Second Indicator Subfields
LEADER 00000cam a2200000 a 4500
001 in00005738136
005 20260326162525.7
006 m o d
007 cr cnu---unuuu
008 090611s2008 cauaf ob 001 0 eng d
040 |a N$T  |b eng  |e pn  |c N$T  |d OCLCQ  |d CUS  |d IDEBK  |d E7B  |d OCLCQ  |d OPELS  |d OCLCQ  |d OPELS  |d OCLCF  |d KUK  |d YDXCP  |d OCLCQ  |d KIJ  |d MERER  |d OCLCQ  |d OCLCO  |d OCLCQ  |d ANS  |d WYU  |d ESU  |d OCLCQ  |d YOU  |d OCLCQ  |d SOI  |d OCLCQ  |d VT2  |d OCLCO  |d LEAUB  |d WURST  |d CUV  |d OCLCO  |d UKDOC  |d OCLCA  |d OCLCO  |d OCL  |d OCLCQ  |d OCLCO  |d OCLCL  |d OCLCQ  |d OCLCL  |d UKBOL  |d ZI0  |d EBLCP  |d DEBSZ 
015 |a GBA890530  |2 bnb 
016 7 |a 014673817  |2 Uk 
019 |a 646802938  |a 712982258  |a 808777999  |a 809731015  |a 816324726  |a 823121470  |a 823843454  |a 823912040  |a 824099227  |a 824154639  |a 846753097 
020 |a 9780080922072  |q (electronic bk.) 
020 |a 0080922074  |q (electronic bk.) 
020 |a 0123743788  |q (electronic bk.) 
020 |a 9780123743787  |q (electronic bk.) 
020 |a 1282287311 
020 |a 9781282287310 
035 |a (OCoLC)388340291  |z (OCoLC)646802938  |z (OCoLC)712982258  |z (OCoLC)808777999  |z (OCoLC)809731015  |z (OCoLC)816324726  |z (OCoLC)823121470  |z (OCoLC)823843454  |z (OCoLC)823912040  |z (OCoLC)824099227  |z (OCoLC)824154639  |z (OCoLC)846753097 
050 4 |a QP601  |b .M49eb v. 448 
060 4 |a W1  |b ME9615K v.448 2008 
060 4 |a QU 25  |b R6267 2008 
072 7 |a SCI  |x 049000  |2 bisacsh 
072 7 |a PSAK, PSD  |2 bicssc 
082 0 4 |a 572.88  |2 22 
049 |a TXAM 
245 0 0 |a RNA turnover in eukaryotes :  |b Nucleases, pathways and analysis of mRNA decay /  |c edited by Lynne E. Maquat, Megerditch Kiledjian. 
246 3 0 |a Nucleases, pathways and analysis of mRNA decay 
260 |a San Diego, California ;  |a London :  |b Academic Press/Elsevier,  |c ©2008. 
300 |a 1 online resource (xlviii, 607 pages, 5 unnumbered pages of plates) :  |b illustrations (some color) 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
347 |a data file 
490 1 |a Methods in enzymology,  |x 0076-6879 ;  |v v. 448 
504 |a Includes bibliographical references and indexes. 
505 0 |a Analysis of mRNA decapping / S.W. Liu [and others] -- A kinetic assay to monitor RNA decapping under single- turnover conditions / B.N. Jones [and others] -- Purification and analysis of the decapping activator Lsm1p-7p-Pat1p complex from yeast / S. Tharun -- Reconstitution of recombinant human LSm complexes for biochemical, biophysical, and cell biological studies / B.L. Zaric and C. Kambach -- Regulated deadenylation in vitro / A.C. Goldstrohm, B.A. Hook and M. Wickens -- Cell-free deadenylation assays with Drosophila embryo extracts / M. Jeske and E. Wahle -- Measuring CPEB-mediated cytoplasmic polyadenylation-deadenylation in Xenopus laevis oocytes and egg extracts / J.H. Kim and J.D. Richter -- The preparation and applications of cytoplasmic extracts from mammalian cells for studying aspects of mRNA decay / K.J. Sokoloski, J. Wilusz and C.J. Wilusz -- In vitro assays of 5' to 3'-exoribonuclease activity / O. Pellegrini [and others] -- Reconstitution of RNA exosomes from human and Saccharomyces cerevisiae cloning, expression, purification, and activity assays / J.C. Greimann and C.D. Lima -- Biochemical studies of the mammalian exosome with intact cells / G. Schilders and G.J. Pruijn -- Determining in vivo activity of the yeast cytoplasmic exosome / D. Schaeffer [and others] -- Approaches for studying PMR1 endonuclease-mediated mRNA decay / Y. Otsuka and D.R. Schoenberg -- Methods to determine mRNA half-life in Saccharomyces cerevisiae / J. Coller -- mRNA Decay analysis in Drosophila melanogaster drug-induced changes in glutathione S-transferase D21 mRNA stability / B. Akgul and C.P. Tu -- Measuring mRNA stability during early Drosophila embryogenesis / J.L. Semotok [and others] -- Messenger RNA half-life measurements in mammalian cells / C.Y. Chen, N. Ezzeddine and A.B. Shyu -- Trypanosomes as a model to investigate mRNA decay pathways / S. Archer [and others] -- Cell type-specific analysis of mRNA synthesis and decay in vivo with uracil phosphoribosyltransferase and 4-thiouracil / M.D. Cleary -- Analysis of cytoplasmic mRNA decay in Saccharomyces cerevisiae / D.O. Passos and R. Parker -- Transcriptome targets of the exosome complex in plants / D. Belostotsky -- Sensitive detection of mRNA decay products by use of reverse-ligation-mediated PCR (RL-PCR) / T. Grange -- Tethering assays to investigate nonsense-mediated mRNA decay activating proteins / N.H. Gehring, M.W. Hentze and A.E. Kulozik -- Assays for determining poly(A) tail length and the polarity of mRNA decay in mammalian cells / E.L. Murray and D.R. Schoenberg -- Analyzing P-bodies in Saccharomyces cerevisiae / T. Nissan and R. Parker -- Real-time and quantitative imaging of mammalian stress granules and processing bodies / N. Kedersha [and others] -- Cell biology of mRNA decay / D. Grunwald, R.H. Singer and K. Czaplinski. 
588 0 |a Print version record. 
520 |a Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements, responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. The control of biological processes, such as cellular growth and differentiation, is dependent on how the genetic material within a cell is expressed. The cellular physiology of mRNA-including mRNA processing, transport, localization, and turnover-is central to the process of gene expression. Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway. Expert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover which are central to the process of gene expression. Offers step-by-step lab instructions including necessary equipment and reagents. 
650 0 |a Messenger RNA. 
650 0 |a Genetic regulation. 
650 0 |a Eukaryotic cells. 
650 0 |a Nucleases. 
650 1 2 |a RNA Precursors 
650 1 2 |a RNA Stability  |x physiology 
650 2 |a Gene Expression Regulation 
650 2 |a Eukaryotic Cells 
650 2 2 |a Eukaryotic Cells  |x metabolism 
650 2 2 |a mRNA Cleavage and Polyadenylation Factors 
650 2 2 |a RNA, Messenger  |x metabolism 
650 6 |a Régulation génétique. 
650 6 |a Cellules eucaryotes. 
650 6 |a Nucléases. 
650 7 |a SCIENCE  |x Life Sciences  |x Molecular Biology.  |2 bisacsh 
650 7 |a Eukaryotic cells  |2 fast 
650 7 |a Genetic regulation  |2 fast 
650 7 |a Messenger RNA  |2 fast 
650 7 |a Nucleases  |2 fast 
653 0 0 |a rna 
653 0 0 |a metabolisme 
653 0 0 |a metabolism 
653 0 0 |a degradatie 
653 0 0 |a degradation 
653 1 0 |a Metabolic Processes 
653 1 0 |a Metabole processen 
655 7 |a Electronic books.  |2 local 
655 2 |a Laboratory Manual 
655 7 |a Laboratory manuals  |2 fast 
655 7 |a Laboratory manuals.  |2 lcgft 
655 7 |a Manuels de laboratoire.  |2 rvmgf 
700 1 |a Maquat, Lynne,  |e editor. 
700 1 |a Kiledjian, Megerditch,  |e editor. 
710 2 |a ScienceDirect (Online service) 
776 0 8 |i Print version:  |t RNA turnover in eukaryotes.  |d San Diego, California ; London : Academic Press/Elsevier, ©2008  |z 0123743788  |w (OCoLC)228364730 
830 0 |a Methods in enzymology ;  |v v. 448.  |x 0076-6879 
856 4 0 |u http://proxy.library.tamu.edu/login?url=https://www.sciencedirect.com/science/bookseries/00766879/448  |z Connect to the full text of this electronic book  |t 0 
955 |a Elsevier ScienceDirect 2026-2027 
994 |a 92  |b TXA 
999 f f |i 9d5394a4-8282-4d59-a3c1-a068216f8503  |s d9bbc49a-0e39-4a5d-be3c-368bc2573ee1  |t 0 
952 f f |a Texas A&M University  |b College Station  |c Electronic Resources  |s www_evans  |d Available Online  |t 0  |e QP601 .M49eb v. 448  |h Library of Congress classification 
998 f f |a QP601 .M49eb v. 448  |t 0  |l Available Online