http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Adenylyl cyclase, G proteins, and guanylyl cyclase
1 online resource (xxx, 512 pages) : illustrations
This volume emphasizes methods for the assay, purification, and characterization of adenylyl cyclases, guanine nucleotide-dependent regulatory proteins (G proteins), and guanylyl cyclases. Key Features * Adenylyl cyclase: determination of activity * Preparation of materials useful in purification of components of hormonally responsive cyclase systems * Purification guanine nucleotide-dependent regulatory proteins * Purification and characterization of g proteins: labeling and quantitating of G proteins * Reconstitution guanylyl cyclase: assay * Preparation of materials used for study of the cyclase * Purification * Regulation.
Assay of adenylyl cyclase catalytic activity / Roger A. Johnson and Yoram Salomon -- Cellular responsiveness to hormones and neutrotransmitters: Conversion of [3H]Adenine to [3H]cAMP in cell monolayers, cell suspensions, and tissue slices / Yoram Salomon -- Preparation of [alpha]-32P-Labeled nucleoside triphosphates, nicotinamide adenine dinucleotide, and cyclic nucleotides for use in determining adenylyl and guanylyl cyclases and cyclic nucleotide phosphodiesterase / Timothy F. Walseth [and others] -- Synthesis of forskolin-agarose affinity matrices / Thomas Pfeuffer -- High-affinity binding sites for [3H]forskolin / Antonio Laurenza and Kenneth B. Seamon -- Purification and characterization of calmodulin-sensitive adenylyl cyclase from bovine brain / Ping Wang and Daniel R. Storm -- Purification of adenylyl cyclase from heart and brain / Elke Pfeuffer [and others] -- Calmodulin-mediated adenylyl cyclase from equine sperm / William A. Toscano, Jr. and Mary K. Gross -- Isolation of polyclonal antibodies against bovine brain calmodulin-sensitive adenylyl cyclases / Gary B. Rosenberg [and others] -- Characteristics and use of monoclonal antibodies to various forms of adenylyl cyclase / Stefan Mollner and Thomas Pfeuffer -- Purification of soluble form of adenylyl cyclase from testes / Theodor Braun -- Purification and assay of cell-invasive form of calmodulin-sensitive adenylyl cyclase from Bordetella pertussis / H. Robert Masure [and others] -- Purification and characterization of adenylyl cyclase from Bacillus anthracis / Stephen H. Leppla -- Preparation and application of GTP-agarose matrices / Elke Pfeuffer and Thomas Pfeuffer -- Purification of G proteins / Juan Codina [and others] -- Preparation of guanine nucleotide-free G proteins / Kenneth M. Ferguson and Tsutomu Higashijima -- Purification of recombinant Gs[alpha] / Michael P. Graziano [and others] -- Purification of recombinant G1[alpha] and Go[alpha] proteins from Escherichia coli / Maurine E. Linder and Alfred G. Gilman -- Synthetic peptide antisera with determined specificity for G protein [alpha] or [beta] subunits / Susanne M. Mumby and Alfred G. Gilman -- Quantitation and purification of ADP-ribosylation factor / Richard A. Kahn -- Soluble guanine nucleotide-dependent ADP-ribosylation factors in activation of adenylyl cyclase by cholera toxin / Joel Moss [and others] -- ADP-ribosylation of G proteins with pertussis toxin / Gregory S. Kopf and Marilyn J. Woolkalis -- Cholera toxin-catalyzed [32P]ADP-ribosylation of proteins / D. Michael Gill and Marilyn J. Woolkalis -- Photoaffinity labeling of GTP-binding proteins / Rolf Thomas and Thomas Pfeuffer.
Identification of receptor-activated G proteins with photoreactive GTP analog, [[alpha]-32P]GTP azidoanilide / Stefan Offermanns [and others] -- Quantitative immunoblotting of G-protein subunits / Donna J. Carty [and others] -- Assay of g-protein [beta][gamma]-subunit complex by catalytic support of ADP-ribosylation of Go[alpha] / Patrick J. Casey [and others] -- Tryptophan fluorescence of G proteins: Analysis of guanine nucleotide binding and hydrolysis / Tsutomu Higashijima and Kenneth M. Ferguson -- Reconstitution of receptors and G proteins in phospholipid vesicles / Richard A. Cerione and Elliott M. Ross -- Assay of guanylyl cyclase catalytic activity / Steven E. Domino [and others] -- Preparative polyacrylamide gel electrophoresis apparatus for purification of guanylyl cyclase / Alexander Mülsch and Rupert Gerzer -- Detergent interactions and solubilization techniques for membrane guanylyl cyclase / Arnold A. White and Pushkaraj J. Lad -- Purification of membrane form of guanylyl cyclase / Chodavarapu S. Ramarao and David L. Garbers -- Purification of heme-containing soluble guanylyl cyclase / Alexander Mülsch and Rupert Gerzer -- Preparation of soluble guanylyl cyclase from bovine lung by immunoaffinity chromatography / Peter Humbert [and others] -- Immunoaffinity purification of soluble guanylyl cyclase / Scott A. Waldman [and others] -- Copurification of atrial natriuretic peptide receptor and particulate guanylyl cyclase / Scott A. Waldman [and others] -- Copurification of atrial natriuretic factor receptors and guanylyl cyclase from adrenal cortex / Tadashi Inagami [and others] -- Molecular cloning of membrane forms of guanylyl cyclase / Sujay Singh and David L. Garbers -- Radiation-inactivation analysis of multidomain proteins: The case of particulate guanylyl cyclase / Michel Potier [and others] -- Identification of atrial natriuretic peptide receptors in cultured cells / Dale C. Leitman [and others] -- Evaluating atrial natriuretic peptide-induced cGMP production by particulate guanylyl cyclase stimulation in Vitro and in Vivo / Pavel Hamet and Johanne Tremblay -- Phosphorylation and dephosphorylation of sea urchin sperm cell guanylyl cyclase / J. Kelley Bentley -- Calcium-regulated guanylyl cyclases from Paramecium and Tetrahymena / Joachim E. Schultz and Suzanne Klumpp.
edited by Roger A. Johnson, Jackie D. Corbin.
Includes bibliographical references and indexes.