rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Mitosis and meiosis
  name:
    name part: Rieder, Conly L.
  name:
    name part: ScienceDirect (Online service)
  type of resource: text
  genre: bibliography
  genre: Electronic books.
  origin info:
    place:
      place term: cau
    place:
      place term: San Diego
    publisher: Academic Press
    date issued: ©1999
    date issued: 1999
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource (xv, 489 pages, 12 unnumbered pages of plates) : illustrations (some color)
  abstract: Mitosis and Meiosis details the wide variety of methods currently used to study how cells divide as yeast and insect spermatocytes, higher plants, and sea urchin zygotes. With chapters covering micromanipulation of chromosomes and making, expressing, and imaging GFP-fusion proteins, this volume contains state-of-the-art "how to" secrets that allow researchers to obtain novel information on the biology of centrosomes and kinetochores and how these organelles interact to form the spindle. Chapters Contain Information On: * How to generate, screen, and study mutants of mitosis in yeast, fungi, and flies * Techniques to best image fluorescent and nonfluorescent tagged dividing cells * The use and action of mitoclastic drugs * How to generate antibodies to mitotic components and inject them into cells * Methods that can also be used to obtain information on cellular processes in nondividing cells
  table of contents: Front Cover; Methods in Cell Biology, Volume 61; Copyright Page; Contents; Contributors; Preface; Chapter 1. Isolation of Centrosomes from Drosophila Embryos; I. Introduction; II. Isolation of Centrosomes from Drosophila Embryos; III. Assays for Microtubule Nucleation by Isolated Centrosomes; IV. Conclusions; References; Chapter 2. Studying the Composition and Function of Centrosomes in Vertebrates; I. Introduction; II. Isolation of Centrosomes from Animal Cells; III. Preparation of Immunological Probes from Isolated Centrosomes; IV. Ultrastructural Analysis of Isolated Centrosomes.
  table of contents: v. Biochemical Composition of CentrosomesVI. Functional Assays of Isolated Centrosomes; VII. Prospects; References; Chapter 3. Isolation of Centrosomes from Spisulu solidissima Oocytes; I. Introduction; II. Obtaining Organisms; III. Isolation and Activation of Spisulu solidissima Oocytes; IV. Preparation of Oocyte Lysates; V. Preparation of Microtubule Protein; VI. Isolation of Centrosomes from Activated Oocyte Lysates; VII. lmmunofluorescence of Centrosomes and Asters; VIII. Electron Microscopy of Asters and Centrosomes; IX. Summary; References.
  table of contents: Chapter 4. Methods for in Situ Localization of Proteins and DNA in the Centromere-Kinetochore ComplexI. Introduction; II. In Situ Localization of Proteins: Indirect Immunofluorescence; III. In Situ Localization of Proteins: Immunogold EM; IV. Fluorescent in Situ Hybridization Using DNA Satellite Probes; V. Combination Staining: DNA/Protein; VI. Specialized Techniques; References; Chapter 5. Three-Dimensional Transmission Electron Microscopy and Its Application to Mitosis Research; I. Introduction; II. Resolution and Choosing between Tomography and Serial Sections; III. Electron Tomography.
  table of contents: IV. Serial Section ReconstructionV. Analysis and Display of 3D Reconstructions; VI. Software Packages; VII. Summary and Conclusions; References; Chapter 6. Enlightening Mitosis: Construction and Expression of Green Fluorescent Protein Fusion Proteins; I. Introduction: Visualizing the Molecular Anatomy of the Spindle; II. Fluorescence Properties of GFP; III. Strategies for Constructing Fusion Proteins; IV. Expression in Mammalian Cells; References; Chapter 7. Recombinant p50/Dynamitin as a Tool to Examine the Role of Dynactin in Intracellular Processes; I. Introduction.
  table of contents: II. Production of Recombinant p50/DynamitinIII. Disruption of the Dynactin Complex by p50/Dynamitin in Xenopus Egg Extracts; IV. Disruption of Spindle Poles Using p50/Dynamitin; References; Chapter 8. In Vitro Assays for Studying Saccharomyces cerevisiae Kinetochore Activity; I. Introduction; II. Microtubule-Binding Assays for S. cerevisiae Kinetochores; Ill. Band Shift Assay for the Kinetochore Complex; References; Chapter 9. Fluorescent Speckle Microscopy of Spindle Microtubule Assembly and Motility in Living Cells; I. lntroduction.
  note: edited by Conly L. Rieder.
  note: Includes bibliographical references (pages 464-472) and index.
  subject:
    topic: Cell division
  subject:
    topic: Mitosis
  subject:
    topic: Meiosis
  subject:
    topic: Cell Division
  subject:
    topic: Cell Fractionation
    topic: methods
  subject:
    topic: Meiosis
  subject:
    topic: Mitosis
  subject:
    topic: Mitose
  subject:
    topic: Méiose
  subject:
    topic: Cellules
    topic: Division
  subject:
    topic: SCIENCE
    topic: Life Sciences
    topic: Developmental Biology
  subject:
    topic: Cell division
  subject:
    topic: Meiosis
  subject:
    topic: Mitosis
  subject:
    topic: Celdeling
  subject:
    topic: Meiose
  subject:
    topic: Citologia
  classification: QH605.2 .M54 1999eb
  classification: 571.8/44
  classification: W1 ME9615D v.61 1999
  classification: QH 605 M6835 1999
  related item:
    title info:
      title: Mitosis and meiosis
    origin info:
      publisher: San Diego : Academic Press, ©1999
    identifier: (DLC) 99172833
    identifier: (OCoLC)40513158
  related item:
    title info:
      title: Methods in cell biology ; v. 61
  identifier: 9780080859590
  identifier: 0080859593
  identifier: 0125441630
  identifier: 9780125441636
  identifier: 1281755583
  identifier: 9781281755582
  identifier: http://proxy.library.tamu.edu/login
  record info:
    record content source: N$T
    record creation date: 090114
    record change date: 20260326160124.3
    record identifier: in00005735751
    language of cataloging:
      language term: eng