rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Membrane protein purification and crystallization
    sub title: a practical guide
  name:
    name part: Jagow, Gebhard von.
  name:
    name part: Schägger, Hermann.
  name:
    name part: ScienceDirect (Online service)
  type of resource: text
  genre: bibliography
  genre: Electronic books.
  origin info:
    place:
      place term: ne
    place:
      place term: Amsterdam
    place:
      place term: Boston
    publisher: Academic Press
    date issued: ©2003
    date issued: 2002
    edition: 2nd ed.
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource (xviii, 316 pages) : illustrations (some color)
  abstract: This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Key features * Provides general guidelines and strategies for isolation and crystallization of membrane proteins * Gives detailed protocols that have wide application, and low specialized equipment needs * Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins * Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes * Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization * Presents a comprehensive guide to 2D-crystallization of membrane proteins.
  table of contents: I. Strategies and Techniques -- Purification Strategies for Membrane Proteins. -- Chromatographic Techniques and Basic Operations in Membrane Protein Purification. -- Production and purification of recombinant membrane proteins. -- SDS Electrophoresis Techniques. -- Blue-Native Electrophoresis. -- Preparative Isoelectric Focussing. -- Membrane protein crystallization. -- II. Discussion of Selected Isolation Protocols -- Lipid Dependent Inactivation and Reactivation of Bovine complex III. -- Purification of affinity-epitope tagged G-protein coupled receptors. -- Purification of NhaA, the Na+/H+ Antiporter of Escherichia coli for 3D or 2D -- Crystallization. -- Purification of the yeast cytochrome bc1 complex. -- -- III. Crystallization of Membrane Proteins -- Antibody fragment-mediated crystallization of membrane proteins. -- Crystallization of Wolinella succinogenes quinol:fumarate reductase. -- Ba3-type cytochrome-c oxidase from Thermus thermophilus: -- purification, crystallization, and crystal transformation. -- Two-dimensional crystallization of membrane proteins: a practical guide. -- In cubo crystallization of membrane proteins.
  note: edited by Carola Hunte and Gebhard von Jagow and Hermann Schagger.
  note: Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL
  note: digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL
  note: Includes bibliographical references and index.
  subject:
    topic: Membrane proteins
    topic: Purification
  subject:
    topic: Membrane Proteins
    topic: isolation & purification
  subject:
    topic: SCIENCE
    topic: Life Sciences
    topic: Anatomy & Physiology
  subject:
    topic: SCIENCE
    topic: Life Sciences
    topic: Biochemistry
  subject:
    topic: Membrane proteins
    topic: Purification
  subject:
    topic: Proteínas
  subject:
    topic: Membranas (biologia)
  subject:
    topic: Bioquímica
  classification: QP552.M44 P73 2003eb
  classification: 572/.696
  classification: QU55 HUN
  related item:
    title info:
      title: Membrane protein purification and crystallization
    origin info:
      publisher: Amsterdam ; Boston : Academic Press, ©2003
      edition: 2nd ed.
    identifier: (DLC) 2002100070
    identifier: (OCoLC)54400634
  related item:
    identifier: (DLC) 2002100070
    identifier: (OCoLC)54400634
  identifier: 9780123617767
  identifier: 0123617766
  identifier: 9780080536170
  identifier: 0080536174
  identifier: 89772:89772 Elsevier Science & Technology
  identifier: http://proxy.library.tamu.edu/login
  record info:
    record content source: OPELS
    record creation date: 070802
    record change date: 20260326145834.1
    record identifier: in00005731167
    language of cataloging:
      language term: eng