rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: DNA microarrays
  name:
    name part: Kimmel, Alan R.
    role:
      role term: editor.
  name:
    name part: Oliver, Brian
    name part: 1959-
    role:
      role term: editor.
  name:
    name part: ScienceDirect (Online service)
  type of resource: text
  genre: bibliography
  genre: Electronic books.
  origin info:
    place:
      place term: cau
    place:
      place term: San Diego, California
    publisher: Elsevier/Academic Press
    date issued: ©2006
    date issued: 2006
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource (parts A-B) : illustrations.
  abstract: Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results. Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshooting techniques continue into Part B. These techniques are well rooted in traditional molecular biology and while they require traditional care, a researcher that can reproducibly generate beautiful Northern or Southern blots should have no difficulty generating beautiful array hybridizations. Data management is a more recent problem for most biologists. The bulk of Part B provides a range of techniques for data handling. This includes critical issues, from normalization within and between arrays, to uploading your results to the public repositories for array data, and how to integrate data from multiple sources. There are chapters in Part B for both the debutant and the expert bioinformatician. Provides an overview of platforms Includes experimental design and wet bench protocols Presents statistical and data analysis methods, array databases, data visualization and meta analysis.
  table of contents: Part A (v. 410) Array platforms and wet-bench protocols -- Part B (v. 411) Databases and statistics.
  table of contents: Section I Array Platforms -- Chapter 1: The Affymetrix GeneChipreg Platform: An Overview -- Introduction -- GeneChip Microarrays, a Flexible Platform -- Array Manufacturing -- Array Design -- Target Preparation -- GeneChip Instrument Components and Associated Assay Steps -- Image and Data Analysis -- Current Applications -- Advancing the Future of Genomics -- Acknowledgments -- References -- Chapter 2: The Agilent In Situ-Synthesized Microarray Platform -- Introduction -- Technology -- Applications -- Methods Descriptions -- Array Design -- Sample Isolation, Labeling, and Quality Control -- Array Hybridization and Scanning -- Data Extraction -- Array Design -- Process Description -- Sample Isolation, Labeling, and Quality Control -- Array Hybridization and Scanning -- Data Extraction -- References -- Chapter 3: Illumina Universal Bead Arrays -- Introduction -- Material and Methods -- Results and Discussion -- Conclusion -- Acknowledgments -- References -- Chapter 4: Microarray Oligonucleotide Probes -- The Case for Oligonucleotide Probes -- Considerations for Oligonucleotide Probe Design -- Microarray Production and Hybridization Protocols -- Practical Considerations in Probe Sequence Design, a Case Study -- Employment and Postprocessing -- Conclusion -- Locations of Probe Sequence Target Regions: Discrimination of Highly Similar Targets -- In Situ Synthesis vs Deposition of Presynthesized Oligonucleotides -- Thermodynamic Modeling of Microarray Probe Hybridization -- Outlook -- Supplement -- Acknowledgments -- References -- Chapter 5: Automated Liquid Handling and High-Throughput Preparation of Polymerase Chain Reaction-Amplified DNA for Microarray Fabrication -- Introduction -- Overview -- Methods -- Summary -- Acknowledgments -- References -- Chapter 6: The Printing Process: Tips on Tips -- Introduction -- The Printing Process and Equipment -- Printing Pin Technology Options -- Critical Parameters and Troubleshooting -- Quality Control Testing -- Future Developme…
  note: edited by Alan Kimmel & Brian Oliver.
  note: Includes bibliographical references and indexes.
  subject:
    topic: DNA microarrays
  subject:
    topic: DNA
  subject:
    topic: Oligonucleotide Array Sequence Analysis
    topic: methods
  subject:
    topic: DNA
  subject:
    topic: Gene Expression Profiling
    topic: methods
  subject:
    topic: Puces à ADN
  subject:
    topic: ADN
  subject:
    topic: DNA
  subject:
    topic: DNA microarrays
  subject:
    topic: dna
  subject:
    topic: dna libraries
  subject:
    topic: dna probes
  subject:
    topic: genexpressie
  subject:
    topic: gene expression
  subject:
    topic: rna
  subject:
    topic: Nucleic Acids
  classification: QP601 .C733
  classification: 572.8636
  classification: W1 ME9615K v. 411 etc. 2006
  classification: QU 450 D6293 2006
  related item:
    title info:
      title: DNA microarrays
    origin info:
      publisher: San Diego, California : Elsevier/Academic Press, ©2006
    identifier: (OCoLC)71231099
  related item:
    title info:
      title: Methods in enzymology ; v. 410
  related item:
    title info:
      title: Methods in enzymology ; v. 411
  identifier: 9780080464657
  identifier: 0080464653
  identifier: 0121828158
  identifier: 9780121828158
  identifier: 0121828166
  identifier: 9780121828165
  identifier: http://proxy.library.tamu.edu/login
  record info:
    record content source: AZU
    record creation date: 060911
    record change date: 20260326145031.8
    record identifier: in00005730354
    language of cataloging:
      language term: eng