rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Evaluation of White Blood Cell DNA Methylation as a Tool to Predict DNA Methylation of the Adrenal Axis in Prenatally Stressed, Mature Brahman Cows
  name:
    name part: Collins, Haley Claire
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: Riley, David
    role:
      role term: thesis advisor.
  name:
    name part: Texas A&M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2023
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource.
  abstract: This dissertation project evaluated whether methylation of DNA of peripheral white blood cells (WBC) is predictive of methylation of DNA in components of the hypothalamic-pituitary-adrenal (HPA) axis of mature Brahman cows; and if so, to what extent. This question was assessed by use of DNA extracted from the WBC, paraventricular nucleus of the hypothalamus, anterior pituitary gland, adrenal cortex, and adrenal medulla of 5-year-old Brahman cows derived from dams that served as a control group or from dams that experienced prenatal transportation stress (PNS). The present study sought to determine if stress endured prenatally would result in long term DNA methylation changes to HPA-axis tissues, and if those changes would be reflected in WBC. A group of 5-year-old Brahman cows having been subjected to prenatal transportation stress (n = 6) were used against a control group of 5-year-old Brahman cows (n = 8) that were not exposed to the same stressor prenatally. Global methylation analyses of HPA-axis tissues to WBC, as well as tissue-to-tissue analyses were conducted using EdgeR software. Results showed tissue specific methylation patterns among the HPA-axis tissues that were not well reflected by WBC. The adrenal cortex and adrenal medulla showed consistency with one another, while the anterior pituitary appeared to be the least affected tissue, with the smallest differences in both control and PNS groups across all comparisons to WBC and other tissues. Otherwise, patterns of methylation were inconsistent when tissues were compared against each other. It is likely the consequences of prenatal stress in mature Brahman cows does not manifest in a global nature. In order to determine consistency between methylation patterns of a tissue and WBC, a change from control to PNS must be apparent and measurable, and to where the patterns of both tissue and WBC correspond in a way that reflects that difference. No such pattern consistency was observed between tissue and WBC, …
  note: by Haley Collins.
  note: "Major Subject: Animal Science"
  note: Includes vita.
  note: Doctor of Philosophy Texas A&M University 2022 https://hdl.handle.net/1969.1/198805
  note: Includes bibliographical references.
  note: Text (Dissertation)
  subject:
    topic: Major Animal Science
  subject:
    topic: DNA methylation
  subject:
    topic: epigenetics
  subject:
    topic: genetics
  subject:
    topic: livestock genetics
  subject:
    topic: stress response
  subject:
    topic: HPA axis
  subject:
    topic: adrenal axis
  subject:
    topic: livestock production
  subject:
    topic: cattle stress response
  subject:
    topic: cattle stress
  subject:
    topic: brahman cattle
  identifier: https://hdl.handle.net/1969.1/198805
  record info:
    record content source: TXA
    record creation date: 250321
    record change date: 20250326141439.9
    record identifier: in00005621469
    language of cataloging:
      language term: eng