rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Elucidating Factors Affecting Biodegradation and Uptake of 6:2 Fluorotelomer Sulfonate (6:2 FTSA) in Phyto/Rhizoremediation
  name:
    name part: Yang, Shih-Hung
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: Chu, Kung-Hui
    role:
      role term: thesis advisor.
  name:
    name part: Texas A&M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2023
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource.
  abstract: 6:2 fluorotelomer sulfonic acid (6:2 FTSA) is one of per- and poly-fluoroalkyl substances that has been widely used in industries and as a key ingredient in aqueous film-forming foams (AFFF). The widespread of 6:2 FTSA in the environment have raised the public attentions and concerns. While biotransformation pathway of 6:2 FTSA has been reported, factors affecting desulfonation and defluorination of 6:2 FTSA remain poorly understood. Moreover, current knowledge on phytoremediation and the role of rhizobacteria on 6:2 FTSA biotransformation is limited. To fill the knowledge gap, the goal of this research to elucidate factors that affect the biotransformation and uptake 6:2 FTSA in phyto/rhizoremediation. The effects of carbon and sulfur sources on the gene expression of Rhodococcus jostii RHA1 which is responsible for the 6:2 FTSA biotransformation were first investigated. While alkane monooxygenase and cytochrome P450 were highly expressed in ethanol-, 1-butanol-, and n-octane-grown RHA1 in sulfur-rich medium, these cultures only defluorinated 6:2 fluorotelomer alcohol (6:2 FTOH) but not 6:2 FTSA, suggesting that the sulfonate group in 6:2 FTSA hinders enzymatic defluorination. In sulfur-free growth media, alkanesulfonate monooxygenase was linked to desulfonation of 6:2 FTSA; while alkane monooxygenase, haloacid dehalogenase, and cytochrome P450 were linked to defluorination of 6:2 FTSA. Four degradation metabolites were confirmed, and one was identified as a tentative metabolite. The potential of phytoremediation for 6:2 FTSA by a model plant Arabidopsis thaliana coupled with bioaugmentation of RHA1 under different nutrient and microbiome conditions were then investigated. A hyperaccumulation of 6:2 FTSA, defined as tissue/soil concentration > 10 and high translocation factor (TF > 3), was observed in plants. However, biotransformation of 6:2 FTSA only occurred under sulfur-limited conditions. Spiking RHA1 enhanced the biotransformation of 6:2 FTSA in soil and prom…
  note: by Shih-Hung Yang.
  note: "Major Subject: Civil Engineering"
  note: Includes vita.
  note: Doctor of Philosophy Texas A&M University 2022 https://hdl.handle.net/1969.1/198638
  note: Includes bibliographical references.
  note: Text (Dissertation)
  subject:
    topic: Major Civil Engineering
  subject:
    topic: 6:2 FTSA
  subject:
    topic: Phyto/rhizoremediation
  subject:
    topic: Biotransformation
  subject:
    topic: Bioaugmentation
  subject:
    topic: Stable isotope probing
  identifier: https://hdl.handle.net/1969.1/198638
  record info:
    record content source: TXA
    record creation date: 241022
    record change date: 20250326141429.9
    record identifier: in00005621254
    language of cataloging:
      language term: eng