rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Proteome, Transcriptome, and Function of Extracellular Vesicles Secreted by Fetal Neural Stem Cells in a Developmental Model of Alcohol Exposure
  name:
    name part: Chung, Dae
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: Miranda, Rajesh
    role:
      role term: thesis advisor.
  name:
    name part: Texas A&M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2023
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource.
  abstract: Prenatal alcohol/ethanol exposure (PAE) can result in Fetal Alcohol Spectrum Disorders (FASD), the leading cause of intellectual disability and growth deficits, and have immediate and lifelong adverse effects on an individual's development and health. When it comes to fetal neural stem cells (NSCs), the teratogenic effects of PAE that lead to FASD may be caused by the reprogramming of NSCs to result in abnormal cell proliferation, growth, differentiation, migration, and maturation. As a mechanism for intercellular communication, extracellular vesicles (EVs) have the potential to contribute to ethanol's dysregulation of NSCs and be a key player within ethanol teratogenesis. For this reason, I examined the protein and RNA cargo and function of EVs derived from fetal NSCs under chronic alcohol exposure. To my knowledge, this is the first study to observe that the proteome and transcriptome of EVs differ from their parents-of-origin NSCs in a dose- and sex-dependent manner, caused by a transfer of unique networks of related proteins and related RNA transcripts. Exposure of untreated EVs to naïve NSCs resulted in decreased oxidative metabolism and S-phase, with EVs derived from ethanol-treated NSCs exhibiting diminished effects. The data suggest a presence of a selective sorting mechanism that controls protein and RNA sequestration from cells into EVs, that is vulnerable to ethanol, altering the cargo content of EVs and potentially reprogramming the functionality of EVs in the neurogenic environment to contribute to the pathology of PAE and FASD. The electronic version of this dissertation is accessible from https://hdl.handle.net/1969.1/198712
  note: by Dae Chung.
  note: "Major Subject: Medical Sciences"
  note: Includes vita.
  note: Doctor of Philosophy Texas A&M University 2022 https://hdl.handle.net/1969.1/198712
  note: Includes bibliographical references.
  note: Text (Dissertation)
  subject:
    topic: Major Medical Sciences
  subject:
    topic: Extracellular vesicle
  subject:
    topic: neural stem cell
  subject:
    topic: proteome
  subject:
    topic: transcriptome
  subject:
    topic: fetal alcohol spectrum disorder
  subject:
    topic: prenatal alcohol exposure
  identifier: https://hdl.handle.net/1969.1/198712
  record info:
    record content source: TXA
    record creation date: 250130
    record change date: 20250326141434.5
    record identifier: in00005621251
    language of cataloging:
      language term: eng