rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: NEXT GENERATION SEQUENCING IN FORENSIC SCIENCE
    sub title: a primer
  name:
    name part: Elkins, Kelly M.
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: Zeller, Cynthia B.
    role:
      role term: author.
  name:
    name part: Taylor & Francis
  type of resource: text
  genre: Electronic books.
  origin info:
    place:
      place term: xx
    place:
      place term: S.l.]
    publisher: CRC PRESS
    date issued: 2021
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource
  abstract: Next Generation Sequencing in Forensic Science: A Primer addresses next generation sequencing (NGS) specific to its application to forensic science. The first part of the book offers a history of human identity approaches, including VNTR, RFLP, STR, and SNP DNA typing. It discusses the history of sequencing for human DNA typing, including Sanger sequencing, SNaPshot, pyrosequencing, and principles of next generation sequencing. The chapters present an overview of the forensically focused AmpliSeq, ForenSeq, Precision ID, PowerSeq, and QIAseq panels for human DNA typing using autosomal, Y and X chromosome STRs and SNPs using the MiSeq FGx and Ion Torrent System. The authors outline the steps included in DNA extraction and DNA quantitation that are performed prior to preparing libraries with the NGS kits. The second half of the book details the implementation of ForenSeq and Precision ID to amplify and tag targets to create the library, enrich targets to attach indexes and adaptors, perform library purification and normalization, pool the libraries, and load samples to the cartridge to perform the sequencing on the instrument. Coverage addresses the operation of the MiSeq FGx and Ion Chef, including creating a sample list, executing wash steps, performing NGS, understanding the run feedback files from the instrument, and troubleshooting. ForenSeq and Precision ID panel data analysis are explained, including how to analyze and interpret NGS data and output graphs and charts. The book concludes with mitochondrial DNA (mtDNA) sequencing and SNPs analysis, including the issue of heteroplasmy. The final chapters review forensic applications of microbial DNA, NGS in body fluid analysis, and challenges and considerations for future applications. FEATURES Focuses on human identification using traditional and NGS DNA typing methods targeting short tandem repeats (STRs) Applies the technology and its application to law enforcement investigations and identity and ancestry single…
  note: Includes bibliographical references and index.
  note: Dr. Kelly Elkins is an Associate Professor of Chemistry at Towson University and a founding editor-in-chief of the Journal of Forensic Science Education. Dr. Cynthia Zeller is an Associate Professor of Chemistry at Towson University.
  subject:
    topic: DNA fingerprinting
  subject:
    topic: Forensic genetics
  subject:
    topic: Nucleotide sequence
  subject:
    topic: Bioinformatics
  subject:
    topic: Computational biology
  subject:
    topic: DNA Fingerprinting
  subject:
    topic: Computational Biology
  subject:
    topic: Empreintes génétiques
  subject:
    topic: Génétique légale
  subject:
    topic: Bio-informatique
  subject:
    topic: SCIENCE / Life Sciences / Genetics & Genomics
  subject:
    topic: SCIENCE / Life Sciences / Biochemistry
  subject:
    topic: Computational biology
  subject:
    topic: Bioinformatics
  subject:
    topic: DNA fingerprinting
  subject:
    topic: Forensic genetics
  subject:
    topic: Nucleotide sequence
  classification: RA1057.55
  classification: 614.1
  related item:
    identifier: (OCoLC)1243967182
  identifier: 9781000449198
  identifier: 100044919X
  identifier: 9781000449259
  identifier: 1000449254
  identifier: 9781003196464
  identifier: 1003196462
  identifier: 0367478935
  identifier: 9780367478933
  identifier: 9781032072043 (pbk.)
  identifier: 9781000449259 Ingram Content Group
  identifier: 9781003196464 Taylor & Francis
  identifier: http://proxy.library.tamu.edu/login
  record info:
    record content source: YDX
    record creation date: 210729
    record change date: 20241106181647.8
    record identifier: in00005517731
    language of cataloging:
      language term: eng