rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Characterization of the Localized Immune Responses at the Single Cell Resolution in Arabidopsis Roots
  name:
    name part: Xu, Lahong
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: He, Ping
    role:
      role term: thesis advisor.
  name:
    name part: Cai, James
    role:
      role term: thesis advisor.
  name:
    name part: Texas A&M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2023
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource.
  abstract: Plant roots are organs with multiple zones and cell types grown underground, which are constantly exposed to various pathogen attacks. Root cells can initiate pattern-triggered immunity (PTI) after recognition of elicitor molecules by cell-surface-localized pattern recognition receptors (PRRs). Elicitors can be pathogen-derived (e.g., MAMPs) or self-derived (e.g, phytocytokines). Recent findings have indicated that different cell types may activate immune responses according to their cell identity; however, how PTI is coordinated in roots is poorly understood. Therefore, tissue- and cell type-specific studies are essential to understand how this sophisticated immune system works in roots. In this research, we employed single cell transcriptomic analysis coupled with high-resolution single cell-imaging to study transcriptional outputs in Arabidopsis thaliana, induced by five different elicitors, including two MAMPs and three phytocytokines. This revealed that the transcriptional responses are treatment-, tissue- and cell type-specific in roots. Moreover, phytocytokines induced stronger immune responses than MAMPs in different root cell types and root zones, suggesting that phytocytokines might be interpreted as a stronger ⁰́₋alarm signal⁰́₊ by the root to amplify defense responses initiated by MAMPs. Interestingly, the root cap has a more robust immune response than the meristematic zone and elongation zone in response to different elicitors, indicating that root caps may act as ⁰́₋sentries⁰́₊ that provide specialized protection to the root meristem cells. Importantly, phytocytokines induce differentiated immune responses at different cell types, which might provide a mechanism for coordinating diverse phytocytokines in activating immunity. Finally, gene regulatory network analysis identified the upstream transcriptional regulators and corresponding targets for each root cell type and revealed that the root cell type-specific transcriptomic responses are strongly cor…
  note: by Lahong Xu.
  note: "Major Subject: Molecular and Environmental Plant Sciences"
  note: Includes vita.
  note: Doctor of Philosophy Texas A&M University 2022 https://hdl.handle.net/1969.1/197798
  note: Includes bibliographical references.
  note: Text (Dissertation)
  subject:
    topic: Major Molecular and Environmental Plant Sciences
  subject:
    topic: Single cell transcriptome
  subject:
    topic: Single cell imaging
  identifier: https://hdl.handle.net/1969.1/197798
  record info:
    record content source: TXA
    record creation date: 240823
    record change date: 20240829173455.8
    record identifier: in00005468379
    language of cataloging:
      language term: eng