rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Gene Editing in Rice and Peanut: Multiplex Editing, Protoplast Validation, and Prime Editing
  name:
    name part: Biswas, Sudip
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: Septiningsih, Endang
    role:
      role term: thesis advisor.
  name:
    name part: Texas A&M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2023
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource.
  abstract: CRISPR-Cas9 is a powerful and versatile genome-editing tool for introducing genetic changes for studying gene expression and improving crops, including rice (Oryza sativa L.) and peanut (Arachis hypogaea L.). Resistant starch is a starch product that is not easily digestible and absorbed in the stomach or small intestine and instead is passed on directly to the large intestine. Cereals high in resistant starch are beneficial to improve human health and reduce the risk of diet-related chronic diseases. It was previously reported through chemical mutagenesis and RNA interference studies that some of the starch branching enzymes (SBEs) may play a major role in developing higher levels of resistant starch in crops. To test the specific roles of all four rice SBE genes in rice, a CRISPR-Cas9 vector construct with four SBE gene sgRNAs was transformed into the U.S. rice variety Presidio using Agrobacterium-mediated transformation. Targeted mutations were identified, and several SBE edited lines showed significantly increased resistant starch content up to 15% higher than the parental cultivar, Presidio. This study demonstrated an example of developing lines with high resistant starch through multiplex CRISPR-Cas9-mediated genome editing. Likewise, improving peanut production and nutrition will require new technologies to enable novel trait development. To test multiplex CRISPR-Cas9 genome editing in peanut, an efficient protoplast editing system was developed to knock out a major allergen gene with the help of an endogenous tRNA-processing system. Peanut protoplast isolation and transformation were successfully optimized and two sgRNAs for an allergen gene, Ara h 2, were designed, tested with in vitro digestion with Cas9, and validated by PEG-mediated transformation in protoplasts, resulting in the identification of knockout mutations. Thus, the protoplast transformation system can serve as a rapid and effective tool for gRNA validation in peanut. Lastly, prime editing, a …
  note: by Sudip Biswas.
  note: "Major Subject: Plant Breeding"
  note: Includes vita.
  note: Doctor of Philosophy Texas A&M University 2022 https://hdl.handle.net/1969.1/197324
  note: Includes bibliographical references.
  note: Text (Dissertation)
  subject:
    topic: Major Plant Breeding
  subject:
    topic: Resistant starch
  subject:
    topic: starch branching enzyme (SBE) genes
  subject:
    topic: diet-related chronic diseases
  subject:
    topic: amylopectin
  subject:
    topic: amylose
  subject:
    topic: multiplex CRISPR-Cas9 genome editing
  subject:
    topic: rice (Oryza sativa)
  subject:
    topic: peanut (Arachis hypogaea L.)
  subject:
    topic: gene editing
  subject:
    topic: CRISPR⁰́₃Cas9
  subject:
    topic: Ara h 2
  subject:
    topic: protoplast
  subject:
    topic: transformation efficiency
  identifier: https://hdl.handle.net/1969.1/197324
  record info:
    record content source: TXA
    record creation date: 240819
    record change date: 20240820145855.5
    record identifier: in00005463595
    language of cataloging:
      language term: eng