rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Light sheet fluorescence microscopy
  name:
    name part: Reynaud, Emmanuel G.
    role:
      role term: editor.
  name:
    name part: Tomancak, Pavel
    role:
      role term: editor.
  type of resource: text
  origin info:
    place:
      place term: gw
    date issued: 2024
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource (416 pages)
  abstract: Light Sheet Fluorescence Microscopy An indispensable guide to a novel, revolutionary fluorescence microscopy technique! Light sheet fluorescence microscopy has revolutionized microscopy, since it allows scientists to perform experiments in an entirely different manner and to record data that had not been accessible before. With contributions from noted experts in the fields of physics, biology, and computer science, Light Sheet Fluorescence Microscopy is a unique guide that offers a practical approach to the subject, including information on the basics of light sheet fluorescence microscopy, instrumentation, applications, sample preparation, and data analysis. Comprehensive in scope, the book is filled with the cutting-edge methods as well as valuable insider tips. Grounded in real-world applications, the book includes chapters from major manufacturers that explores their recent systems and developments. In addition, the book hightlights a discussion of a "do-it-yourself" light sheet microscope, making the technique affordable for every laboratory. This important textbook: Serves as an easy-to-understand introduction to light sheet-based fluorescence Includes numerous tips and tricks for advanced practitioners Provides in-depth information on hardware and software solutions for a straightforward implementation of light sheet fluorescence microscopy in the lab Includes chapters from the major manufacturers including Zeiss, Leica, Lavision Biotech, Phase View, and Asi Aimed at cell biologists, biophysicists, developmental biologists, and neuro-biologists, Light Sheet Fluorescence Microscopy offers a comprehensive overview of the most recent applications of this microscopy technique.
  table of contents: 1 Let There be Light Sheet 1 Pavel Tomank̀ and Emmanuel G. Reynaud -- 1.1 Historical Context of Light Sheet Microscopy - Ultramicroscopy -- 1.2 Light Sheet Imaging Across the Twentieth Century -- 1.3 And here Comes the Flood -- 1.4 The Building of a Community -- References -- 2 Illumination in Light Sheet Fluorescence Microscopy 11 Rory M. Power and Jan Huisken -- 2.1 Introduction -- 2.2 Axial Resolution and Optical Sectioning in Light Sheet Microscopy -- 2.2.1 The Point Spread Function in Fluorescence Microscopy -- 2.2.2 The Point Spread Function in Light Sheet Fluorescence Microscopy -- 2.3 Light Sheet Dimensions -- 2.3.1 Gaussian Optics Description of Beam Focusing (x,z Axes) -- 2.3.2 Methods of Light Sheet Production (y Axis) -- 2.4 Practical Light Sheet Generation -- 2.4.1 Static and Pivoted Light Sheets -- 2.4.2 Scanned Light Sheets -- 2.5 Degradation of the Light Sheet in Tissue -- 2.5.1 Absorption of the Light Sheet in Tissue -- 2.5.2 Refraction of the Light Sheet in Tissue -- 2.5.3 Scattering of the Light Sheet in Tissue -- 2.6 Challenges and Benefits of Light Sheet Modes -- 2.6.1 Parallelization of the Light Sheet -- 2.6.2 Image Artifacts Arising from Light Sheet Illumination -- 2.6.3 Homogeneity of Light Sheet Illumination -- 2.6.4 Robustness and Simplicity of Light Sheet Generation -- 2.6.5 The Merits of Static, Pivoted, and Scanned Light Sheets -- 2.7 Multiphoton Excitation -- 2.7.1 Two-Photon Light Sheets -- 2.7.2 Two-Photon Light Sheet Dimensions -- 2.7.3 Comparison with One-Photon Light Sheet Microscopy -- 2.7.4 Comparison with Laser-Scanning Multiphoton Microscopy -- 2.8 Multi-View Illumination -- 2.9 High-Resolution Imaging -- 2.9.1 Geometric Limitations for High-Resolution Imaging -- 2.9.1.1 Oblique Light Sheets -- 2.9.1.2 Reflected Light Sheets -- 2.9.2 Diffractive Limitations for High-Resolution Imaging -- 2.9.2.1 Bessel Beams -- 2.9.2.2 Axially Swept Beams -- 2.9.2.3 Photophysical Approaches -- 2.10 Conclusions -- References -- 3 A Small Guide …
  table of contents: nality Reduction for Better Analysis -- 7.5.2 Segmentation and Tracking in Light Sheet Data -- 7.5.3 Atlas Registration -- 7.6 Conclusion -- References -- 8 Imaging Molecular Dynamics Using a Light Sheet Microscope 231 Jagadish Sankaran and Thorsten Wohland -- 8.1 Introduction -- 8.2 Fluorescence Techniques Using Light Sheet Illumination -- 8.2.1 Fluorescence Correlation Spectroscopy -- 8.2.2 Fluorescence Recovery After Photobleaching -- 8.2.3 Single-Particle Tracking -- 8.2.4 F̲rster Resonance Energy Transfer -- 8.2.5 Fluorescence Anisotropy Imaging -- 8.2.6 Fluorescence Lifetime Imaging Microscopy -- 8.3 Instrumentation -- 8.3.1 Light Sheet Microscope Configurations -- 8.3.2 Objectives and Cameras -- 8.4 Considerations for Acquisition Parameters -- 8.4.1 Light Sheet Thickness Versus Field of View -- 8.4.2 Field of View Versus Frame Rate -- 8.4.3 Pixel Size Versus Spatial Resolution -- 8.4.4 Pixel Size Versus Field of View and Frame Rate -- 8.4.5 Data Rate -- 8.4.6 Synchronous Versus Asynchronous Read-Out -- 8.4.7 Photobleaching and Phototoxicity -- 8.5 Applications of Fluorescence Techniques Performed Using Light Sheet Microscopes -- 8.5.1 Fluorescence Correlation Spectroscopy -- 8.5.2 Fluorescence Recovery After Photobleaching -- 8.5.3 Single-Particle Tracking -- 8.5.4 F̲rster Resonance Energy Transfer -- 8.5.5 Fluorescence Anisotropy Imaging -- 8.5.6 Fluorescence Lifetime Imaging Microscopy -- 8.6 Concluding Remarks -- References -- 9 Light-Sheet Applications: From Rare Cell Detection to Full Organ Analysis 269 Julien Colombelli, Šbastien Tosi, Alexis Maizel, Linus Manubens Gil, and Jim Swoger -- 9.1 Introduction -- 9.2 3D Imaging of Rare Cells/Events -- 9.2.1 Immunology -- 9.2.2 Cryptococci Infections -- 9.3 Full 3D Imaging and Analysis for Diagnostics -- 9.3.1 Alzheimer's Disease -- 9.3.2 Toward Preclinical Diagnosis Through LSFM Cancer Imaging: Shedding Light on Whole Tumors -- 9.3.2.1 Angiogenesis -- 9.3.2.2 Metastatic Invasion -- 9.4 Population-Based Analys…
  note: edited by Emmanuel G. Reynaud and Pavel Tomancak.
  subject:
    topic: Fluorescence microscopy
  classification: QH212.F55 L54 2024
  classification: 502.82
  identifier: 9783527803910
  identifier: 3527803912
  identifier: 9783527803903
  identifier: 9783527803934
  identifier: 9783527341351
  identifier: http://proxy.library.tamu.edu/login
  record info:
    record content source: NhCcYBP
    record creation date: 240307
    record change date: 20260214210147.3
    record identifier: in00004782303
    language of cataloging:
      language term: eng