rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    non sort: The
    title: Development and Preliminary Use of an In Vitro Test for Susceptibility to Induced Chromosomal Damage
  name:
    name part: Minor, Joseph E., Jr.
    role:
      role term: creator
  name:
    name part: Brickham, John W.
    role:
      role term: degree supervisor.
  type of resource: software, multimedia
  genre: Undergraduate thesis
  origin info:
    place:
      place term: txu
    date issued: 1983
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: electronic
    extent: 1 online resource (12 pages).
  abstract: A new in vitro test for susceptibility to induced chromosomal damage is described. It involves treating primary fibroblast cultures with a known clastogen, bleomycin. The test differs from those previously published in that it makes use of fibroblast cell lines that are also used for standard cytosystematic procedures. Preliminary results from tests carried out on Myotis lucifugus and Peromyscus leucopus are discussed. Data comparing susceptibility to induced chromosomal damage between species and between members of a species are sparce. The studies that have been done (e.g. Hsu et al., 1981; Leonard, et al., 1982) typically use lymphocyte cultures. Phytohemagglutinin stimulated cultures offer only one attempt at obtaining a usable karyotype, while virally transformed lymphoid lines rapidly undergo aneuploid changes. To avoid these problems, the test described here utilizes primary fibroblast cell lines routinely established in the lab for standard cytosystematic work. The test is in the form of an in vitro challenge test: the cells are treated with bleomycin and then harvested and checked for chromosomal aberrations. Bleomycin was chosen as the chemical clastogen (chromosomal breaking agent) since it comes closest to imitating the random chromosomal damage pattern produced by X-rays (Painter, 1982).
  target audience: specialized
  note: Undergraduate thesis written for Program year: 1982-1983
  note: Digitized from print version held at Pickle Center High Density Storage, HDR barcode A14851268103
  note: University Undergraduate Fellows, sponsored by Biology Department.
  note: HDR
  subject:
    topic: induced chromosomal damage
  subject:
    topic: diagnostic testing
  subject:
    topic: Myotis lucifugus
  subject:
    topic: Peromyscus leucopus
  subject:
    topic: primary fibroblast cell lines
  identifier: https://oaktrust.library.tamu.edu/handle/1969.1/CAPSTONE-MinorJ_1983
  record info:
    record creation date: 231116
    record change date: 20240311193112.0
    record identifier: in00004782028