Genetic Manipulation of Aspartate Transcarbamylases from Escherichia coli and Serratia Marcescens.

Bibliographic Details
Main Author: Beck, DeAndra
Format: Thesis Book
Language:English
Published: [College Station, Texas] : Texas A&M University, 1983.
Subjects:
Online Access:Available on OAKTrust.

MARC

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099 |a BeckD 1983 
100 1 |a Beck, DeAndra. 
245 1 0 |a Genetic Manipulation of Aspartate Transcarbamylases from Escherichia coli and Serratia Marcescens. 
264 1 |a [College Station, Texas] :   |b  Texas A&M University,   |c  1983. 
300 |a 1 online resource (32 pages). 
336 |a text   |b  txt   |2  rdacontent 
337 |a computer   |b  c   |2  rdamedia 
338 |a online resource   |b  cr   |2  rdacarrier 
500 |a Undergraduate thesis written for Program year: 1982-1983 
502 |a University Undergraduate Fellows, sponsored by Biochemistry Department. 
520 3 |a Aspartate transcarbamylase (ATCase, EC 2.1.3.2) allosterically modulates de novo pyrimidine biosynthesis in the enteric bacteria. This modulation differs in Escherichia coli and Serratia marcescens, with CTP allosterically inhibiting ATCase from E. coli and activating the enzyme in S. marcescens. The enzyme is composed of two catalytic trimers and three regulatory dimmers encoded by the pyrB and pyrI genes respectively. These adjacent cistrons are organized into a single control region to achieve coordinated biosynthesis of the catalytic and regulatory polypeptide chains necessary to form the holoenzyme. The genes for ATCase from the two organisms may be manipulated by in vitro recombinant DNA techniques. After the structure of the pyrBI genes encoding ATCase are characterized, they may be genetically altered and then transformed back into appropriate strains. The in vivo effects may then be studied. Eventually, DNA sequencing may be done for evaluation of evolutionary trends. 
530 |a Digitized from print version held at Pickle Center High Density Storage, HDR barcode A14851268053 
535 |a HDR 
653 |a ATCase 
653 |a bacteria strains 
653 |a gene encoding 
653 |a genetic alteration 
653 |a pyrB genes 
653 |a pyrI genes 
655 7 |a Undergraduate thesis   |2  local 
700 1 |a Wild, James R.,   |e  degree supervisor. 
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