rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Characterization of Fluffy A. nidulans Mutants Induced by 5-Azacytidine and 4-Nitroquinoline-1-Oxide
  name:
    name part: Valadez, Victoria A.
    role:
      role term: creator
  name:
    name part: Adams, Thomas
    role:
      role term: degree supervisor.
  type of resource: software, multimedia
  genre: Undergraduate thesis
  origin info:
    place:
      place term: txu
    date issued: 1991
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: electronic
    extent: 1 online resource (21 pages).
  abstract: The manner in which the fluF mutation was isolated makes it very interesting because it suggests that it may arise from an epigenetic defect. 5-Azacytidine (5-AC) treatment results in a high frequency of conversion of wild type to fluffy suggesting that the mutation may arise from the demethylation of a sequence that is normally methylated. If true, this would be the first example of an epigenetic event altering the development in a filamentous fungal system. It is also important to note that A. nidulans is characterized by a negligible level of methylation, if methylation exists at all (2). However, 5-AC has been shown to induce permanent heritable phenotypic changes in a variety of eukaryotic systems. Further evidence shows that control of gene expression in more complex systems is related to methylation of cytosines in the DNA and that these methylayion patterns are inherited (8). It would be interesting to characterize fluF and determine the mechanisms involved in its expression. Other chemical mutagens may be used to induce fluffy mutants as well. Although such treatment could result in many types of mutants, visual screening may be used to isolate only those mutants with the fluffy appearance. In these experiments two mutagens, 5-AC and 4-Nitroquinoline-1- (NQO), were used to induce and isolate fluffy mutants. 5-AC is an analog of cytosine that has a nitrogen atom substituted for a carbon at the site of methylation. When incorporated into the DNA, even at marginal levels, it inhibits eukaryotic DNA methyl transferase, thus causing extensive hypomethylation of the DNA. NQO is a classical mutagen that has been used extensively in A. nidulans because it induces mutation primarily in GC base pairs, and is easy to inactivate (3). In these studies, mutants were divided into two groups for the purpose of analyzing the 5-AC and NQO mutants separately. The fluffy strains were then divided into groups based on whether or not conidiation was completely abolished. Further…
  target audience: specialized
  note: Undergraduate thesis written for Program year: 1990/1991
  note: Digitized from print version held at Pickle Center High Density Storage, barcode 24829740
  note: University Undergraduate Fellow, sponsored by Biology Department.
  note: HDR
  subject:
    topic: acoD mutation
  subject:
    topic: ascomycetous fungi
  subject:
    topic: Aspergillus nidulans
  subject:
    topic: conidiation
  subject:
    topic: fluffy mutation
  subject:
    topic: fungi
  subject:
    topic: growth rate
  subject:
    topic: mutagebs
  identifier: https://oaktrust.library.tamu.edu/handle/1969.1/CAPSTONE-ValadezV_1991
  record info:
    record creation date: 231116
    record change date: 20240311193059.7
    record identifier: in00004781869