| Abstract: | A great number of tract-tracing techniques are presently available for the exploration of nervous system organizations. One such method relies on the incorporation of horseradish peroxidase (HRP) into the neuron, its transport, and its subsequent localization in other parts of the neuron. The tracer molecule used in this experiment is wheatgerm agglutinin horseradish peroxidase (WGA-HRP). The conjugation with wheatgerm agglutinin allows cell labeling not only to occur in the retrograde but also the anterograde direction. With this capability both the afferents and efferents to the chosen site of HRP injection can be studied. The purpose of this experiment is to assess a technique and its reproducibility in determining the sources of afferents to the hindlimb area (HL) of the parietal (Par) cortex in the brain of test rats. |
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