| Abstract: | Bacillus thuringiensis spores were grown on nonsupplemented, 0.1, 0.5, or 1.0 percent glucose supplemented nutrient agar. Spores were isolated using a density gradient of Angiovist. The spore coats were removed through solubilization with a Tris buffer containing sodium dodecyl sulfate. Soluble versus total protein content for spores from each concentration was then analyzed by a dye-binding technique and the respective ratios were calculated. Sodium dodecyl sulfate polyacrylamide gel electrophoresis further analyzed the soluble and the total spore proteins. |
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