rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    non sort: The
    title: Preparation and Characterization of Reduced Methylated Ribonuclease T1
  name:
    name part: Laurents, Douglas V.
    role:
      role term: creator
  name:
    name part: Pace, C. N.
    role:
      role term: degree supervisor.
  type of resource: software, multimedia
  genre: Undergraduate thesis
  origin info:
    place:
      place term: txu
    date issued: 1990
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: electronic
    extent: 1 online resource (26 pages).
  abstract: To study the contribution of disulfide bonds to protein stablity, a derivative of ribonuclease T1 (RNase T1) with both cystine residues reduced and methylated has been prepared using an improved procedure adapted from that of Heinrikson [Heinrikson, R.L. (1971) J. Biol. Chern. 246, 4090]. Characterization using urea and thermal denaturation show that the reduced, methylated ribonuclease T1 is about 8 kcal/mol less stable than RNase T1, but is .5 and 1 kcal/mol more stable than reduced carboxyamidomethylated and reduced carboxymethylated RNase T1, respectively. It is suggested that this difference in stability arises from the increase in conformational entropy of the unfolded state and the size and hydrophobicity of the blocking group.
  target audience: specialized
  note: Undergraduate thesis written for Program year: 1989/1990
  note: Digitized from print version held at Pickle Center High Density Storage, barcode 24829692
  note: University Undergraduate Fellows, sponsored by Biochemistry Department.
  note: HDR
  subject:
    topic: conformational entropy
  subject:
    topic: hydrophobicity
  subject:
    topic: protein stability
  subject:
    topic: ribonuclease T1
  subject:
    topic: size
  identifier: https://oaktrust.library.tamu.edu/handle/1969.1/CAPSTONE-LaurentsD_1990
  record info:
    record creation date: 231116
    record change date: 20240301201632.8
    record identifier: in00004780692