| Abstract: | Nerve Growth Factor (NGF) was isolated and purified from the male mouse submaxillary gland and from lyophilized snake venom. Biological activity, isoelectric point and molecular weight were compared. Mouse NGF showed a maximum activity between 4 ng/ml and 35 ng/ml whereas snake NGF showed maximum activity in the 60 -550 ng/ml range. The beta-subunit of mouse NGF has a pI of 9.2 and the snake NGF showed a pI of 8.3 - 8.5. When snake NGF was subjected to SDS gel electrophoresis, the minimum molecular weight was determined to be approximately 10,000 daltons. |
|---|