| Abstract: | In this study, cyclic 3':5'-monophosphate phosphodiesterase from human lung tissue was investigated. After a brief review of the literature, some physical properties of the enzyme were characterized. A Heat Lability study was done, and it was determined that the enzyme was fairly heat stable, with a critical temperature of 39.9°C. The molecular weight was determined by gel filtration and reported as 129,500. This value is lower than other literature values. The enzyme was determined to be labile when subjected to pH values below about 7 to 8. This is in accord with the literature. The pI is reported as less than pH 5, which doesn't fit the literature value of pH 5.3. |
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