Description
| Abstract: | The replication of the telomeres of Borrelia burgdorferi's 16 kb and 49 kb linear plasmids was investigated. Two dimensional agarose gel electrophoresis was used to analyze the replication intermediates of B. burgdorferi in an attempt to determine the mechanism of telomere replication. Oxytricha telomerase assays were used to investigate the possible existence of a telomerase-like enzyme in B. burgdorferi. The two dimensional agarose gels failed to identify specific replication intermediates; the telomerase assay either worked weakly or failed to work. This suggests that i) B. burgdorferi does not depend on telomerase to maintain its telomeres or ii) if telomerase exists, its substrate specificity differs significantly from that of Oxytricha telomerase. |
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| Item Description: | Undergraduate thesis written for Program year: 1992/1993 |
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| Physical Description: | 1 online resource (22 pages).
Digitized from print version held at Pickle Center High Density Storage, barcode 24829590 |
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