Biotechnology explorations : applying the fundamentals /
This exciting volume offers a broad collection of college-level experiments that provides students with a hands -on-understanding of biotechnology and molecular biology, including applications and practical uses. Each investigation includes suggested reading, laboratory language, and analysis questi...
| Main Authors: | , , |
|---|---|
| Format: | eBook |
| Language: | English |
| Language Notes: | English. |
| Published: |
Washington, D.C. :
ASM Press,
©2000.
|
| Subjects: | |
| Online Access: | Connect to the full text of this electronic book |
Table of Contents:
- Laboratory Safety 1
- Fundamentals 9
- Exercise 1 Documentation of Laboratory Experiments 11
- Exercise 2 Use of Equipment 15
- Exercise 3 Preparation of Solutions 23
- Exercise 4 Preparation and Maintenance of Microbial Cultures 31
- Exercise 5 Isolation of DNA by Spooling 41
- Exercise 6 Investigating the Structure and Bond Strength of DNA 45
- Section II Techniques 49
- Exercise 7 Restriction Enzyme Analysis of Lambda DNA 49
- Exercise 8 Miniprep of Plasmid DNA 59
- Exercise 9 Bacterial Transformation 67
- Exercise 10 Amplification of Human DNA by the Polymerase Chain Reaction 75
- Exercise 11 Quantitation of DNA by Spectrophotometry 85
- Exercise 12 Quantitation of DNA by Gel Electrophoresis 91
- Applying the Fundamentals 97
- Section III DNA Cloning 99
- Exercise 13 DNA Ligation and Cloning 99
- Exercise 14 DNA Cloning by Polymerase Chain Reaction 113
- Exercise 15 DNA Cloning for Protein Expression 127
- Section IV Southern Blotting 141
- Exercise 16 Restriction Fragment Length Polymorphism Analysis of Human DNA 141
- Section V Protein Studies 157
- Exercise 17 Quantitation of Protein 157
- Exercise 18 Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Coomassie Blue Staining of Protein 163
- Exercise 19 Western Blotting and Immunodetection 171
- Section VI Gene Transfer 187
- Exercise 20 Transformation of Yeasts 187
- Exercise 21 Transformation of Plants 195
- Section VII Techniques for Studying Evolution and Systematics 203
- Exercise 22 Isolation of Genomic DNA 203
- Exercise 23 Comparison of DNAs from the Five Kingdoms 215
- Exercise 24 Evolution and Diversity through Protein Homologies 219
- Section VIII Internet- and Computer-Assisted Gene Analysis 225
- Exercise 25 Internet Gene Bank Search 225
- Exercise 26 Computer-Assisted Sequence Analysis 233
- Exercise 27 Oligonucleotide Design by Computer 241
- Section IX Advanced Techniques 251
- Exercise 28 DNA Sequencing 251
- Exercise 29 DNA Library Construction 259.