Mass Spectrometry for Lipidomics : Methods and Applications /

Bibliographic Details
Other Authors:	Holčapek, Michal, 1971- (Editor), Ekroos, Kim (Editor)
Format:	eBook
Language:	English
Published:	Newark, NJ : John Wiley & Sons, Inc., 2023.
Subjects:	Lipids > Analysis. Lipids > Spectra. Mass spectrometry.
Online Access:	Connect to the full text of this electronic book

Table of Contents:

Cover
Volume 1
Title Page
Copyright Page
Contents
Preface
Chapter 1 Introduction to Lipidomics
1.1 Preface
1.2 Historical Perspective
1.3 Sampling and Preanalytics
1.4 Reference Materials and Biological Reference Ranges
1.5 Clinical Lipidomics
1.6 Identification and Annotation
1.7 Quantitation
1.8 Lipid Ontology
References
Part I Analytical Methodologies in Lipidomics
Chapter 2 Preanalytics for Lipidomics Analysis
2.1 Safety
2.2 Introduction
2.3 Sample Origin
2.4 Sample Collection
2.5 Tissue Homogenization
2.5.1 Mortar and Pestle
2.5.2 Rotor-Stator
2.5.3 Blender
2.5.4 Potter-Elvehjem
2.5.5 Bead Mill
2.6 Liquid-Liquid Extraction (LLE)
2.6.1 Folch Method
2.6.2 Bligh and Dyer (BD) Method
2.6.3 Modified Folch and Bligh/Dyer (BD) Methods
2.6.4 Rose and Oaklander (RO) Method
2.6.5 Matyash or Methyl-tert-Butyl Ether (MTBE) Method
2.6.6 BUME Method
2.6.7 Alshehry Method
2.6.8 Three-Phase Lipid Extraction (3PLE)
2.7 Resuspension and Solubilization
2.8 Automation
2.9 Tips and Tricks
Acknowledgments
References
Chapter 3 Direct Infusion (Shotgun) Electrospray Mass Spectrometry
3.1 Introduction
3.2 Complexity of Crude Lipid Extracts
3.2.1 Main Lipid Classes in Mammalian Samples
3.2.2 Bond Types as Structural Features
3.2.3 Fatty Acids as the Major Building Blocks
3.2.4 Lipid Species and Double-Bond Series
3.3 Introduction to Mass Spectrometry of Lipids
3.3.1 Annotation of Lipid Structures Analyzed by MS
3.3.2 Isomers
3.3.3 Isobars and the Type-II Isotopic Overlap
3.4 Overview of Direct Infusion MS Workflows
3.5 Sample Preparation
3.5.1 Preanalytics - Sample Stability
3.5.2 Lipid Extraction
3.5.3 Solvents, Additives, and Lipid Concentration
3.5.4 Sample Derivatization
3.6 Direct Infusion
3.7 Mass Spectrometry Analysis
3.7.1 Electrospray Ionization of Lipids
3.7.2 Tandem Mass Spectrometry
3.7.3 Multidimensional MS Shotgun Lipidomics
3.7.4 High-Resolution Mass Spectrometry
3.8 Lipid Identification
3.8.1 Identification by MS/MS
3.8.2 Identification by HRMS
3.8.3 Consideration of Type-II Overlap
3.8.4 Identification Hierarchy
3.8.5 Caveats/Pitfalls
3.9 Lipid Quantification
3.9.1 Internal Standards
3.9.2 Type-I Isotopic Effect
3.9.3 Evaluation and Correction of Isotopic Overlap
3.9.4 Species Response
3.9.5 Calculation of Concentration
3.10 Data Analysis/Software
3.11 Limitations
3.12 Selected Applications
3.12.1 Analysis of Plasma
3.12.2 Analysis of Tissues and Cells
3.12.3 Analysis of Lipid Metabolism
3.13 Outlook
References
Chapter 4 Liquid Chromatography
and Supercritical Fluid Chromatography
Mass Spectrometry
4.1 Introduction
4.2 Lipid Class Separation
4.2.1 Normal-Phase Liquid Chromatography