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Electrophoresis in practice : a guide to methods and applications of DNA and protein separations /

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The fifth edition of this successful text has been revised and expanded, omitting some topics on obsolete DNA electrophoresis. It features a section on electrophoretic micro-methods and on-the-chip electrophoresis. It is geared towards advanced students and professionals and contains extended backgr...

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Bibliographic Details
Main Author: Westermeier, Reiner (Author)
Format: eBook
Language:English
Published: Weinheim, Germany : Wiley-VCH, [2016]
Edition:Fifth edition.
Subjects:
Electrophoresis > Laboratory manuals.
DNA > Separation > Laboratory manuals.
Proteins > Separation > Laboratory manuals.
Electrophoresis.
Electrophoresis
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Front Matter
  • Part I: Fundamentals. Fundamentals
  • Electrophoresis
  • Isotachophoresis
  • Isoelectric Focusing
  • High-Resolution Two-Dimensional Electrophoresis
  • Protein Sample Preparation
  • Protein Detection
  • Blotting
  • Part II: Equipment and Methods. Equipment and Methods
  • Special Laboratory Equipment
  • Consumables
  • Chemicals
  • Method 1: PAGE of Dyes
  • Method 2: Agarose and Immunoelectrophoresis
  • Method 3: Titration Curve Analysis
  • Method 4: Native PAGE in Amphoteric-Buffers
  • Method 5: Agarose IEF
  • Method 6: PAGIEF in Rehydrated Gels
  • Method 7: Horizontal SDS-PAGE
  • Method 8: Vertical PAGE
  • Method 9: Semidry Blotting of Proteins
  • Method 10: IEF in Immobilized pH Gradients
  • Method 11: High-Resolution 2D Electrophoresis
  • Method 12: PAGE of DNA Fragments
  • Appendix: Troubleshooting.