rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Methods in bioengineering
    sub title: systems analysis of biological networks
  title info:
    title: Systems analysis of biological networks
  name:
    name part: Jayaraman, Arul.
  name:
    name part: Hahn, Juergen.
  name:
    name part: Knovel (Firm)
  type of resource: text
  genre: bibliography
  genre: Electronic books.
  origin info:
    place:
      place term: mau
    place:
      place term: Boston [Mass.]
    publisher: Artech House
    date issued: ©2009
    date issued: 2009
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource (xii, 316 pages) : illustrations
  abstract: This practical book is part of the new Artech House Methods in Bioengineering series - volumes designed to offer detailed guidance on authoritative methods for addressing specific bioengineering challenges. Written and edited by recognized experts in the field, each book provides research engineers, scientists, and students with step-by-step procedures, clear examples, and effective ways to overcome problems that may be encountered. This volume focuses on the design of state-of-the art methods for investigating complex biological systems and the development of complex models to analyze the data. Professionals find how-to guidance on experimental approaches for investigating cellular behavior in health and disease.
  table of contents: Methods in Bioengineering: Systems Analysis of Biological Networks; Contents; Chapter 1 Quantitative Immunofluorescence for Measuring Spatial Compartmentation of Covalently Modified Signaling Proteins; 1.1 Introduction; 1.2 Experimental Design; 1.3 Materials; 1.3.1 Cell culture; 1.3.2 Buffers/reagents; 1.3.3 Immunofluorescence reagents; 1.4 Methods; 1.4.1 Cell culture and stimulation for phospho-ERK measurements; 1.4.2 Antibody labeling of phosphorylated ERK (ppERK); 1.4.3 Fluorescence microscopy imaging of ppERK and automated imageanalysis
  table of contents: 1.5 Data Acquisition, Anticipated Results, and Interpretation1.6 Statistical Guidelines; 1.7 Discussion and Commentary; 1.8 Application Notes; 1.9 Summary Points; Acknowledgments; References; Chapter 2 Development of Green Fluorescent Protein-Based Reporter Cell Lines for Dynamic Profiling of Transcription Factor and Kinase Activation; 2.1 Introduction; 2.2 Materials; 2.2.1 Cell and bacterial culture; 2.2.2 Buffers and reagents; 2.2.3 Cloning; 2.2.4 Microscopy; 2.3 Methods; 2.3.1 3T3-L1 cell culture; 2.3.2 Transcription factor reporter development; 2.3.3 Kinase reporter development
  table of contents: 2.4 Application Notes2.4.1 Electroporation of TF reporter plasmids into 3T3-L1 preadipocytes; 2.4.2 Monitoring activation of ERK in HepG2 cells; 2.5 Data Acquisition, Anticipated Results, and Interpretation; 2.6 Discussion and Commentary; 2.7 Summary Points; Acknowledgments; References; Chapter 3 Comparison of Algorithms for Analyzing Fluorescent Microscopy Images and Computation of Transcription Factor Profiles; 3.1 Introduction; 3.2 Preliminaries; 3.2.1 Principles of GFP reporter systems; 3.2.2 Wavelets; 3.2.3 K-means clustering; 3.2.4 Principal component analysis
  table of contents: 3.2.5 Mathematical description of digital images and image analysis3.3 Methods; 3.3.1 Image analysis based on wavelets and a bidirectional search; 3.3.2 Image analysis based on K-means clustering and PCA; 3.3.3 Determining fluorescence intensity of an image; 3.3.4 Comparison of the two image analysis procedures; 3.4 Data Acquisition, Anticipated Results, and Interpretation; 3.4.1 Developing a model describing the relationship between the transcription factor concentration and the observed fluorescence intensity
  table of contents: 3.4.2 Solution of an inverse problem for determining transcription factor concentrations3.5 Application Notes; 3.6 Summary and Conclusions; Acknowledgments; References; Chapter 4 Data-Driven, Mechanistic Modeling of Biochemical Reaction Networks; 4.1 Introduction; 4.2 Principles of Data-Driven Modeling; 4.2.1 Types of experimental data; 4.2.2 Data processing and normalization; 4.2.3 Suitability of models used in conjunction with quantitative data; 4.2.4 Issues related to parameter specification and estimation; 4.3 Examples of Data-Driven Modeling
  note: Arul Jayaraman, Juergen Hahn, editors.
  note: Includes bibliographical references and index.
  subject:
    topic: Bioengineering
  subject:
    topic: Biotechnology
  subject:
    topic: Biological models
  subject:
    topic: System analysis
  subject:
    topic: Bioengineering
    topic: methods
  subject:
    topic: Models, Biological
  subject:
    topic: Systems Analysis
  subject:
    topic: Biotechnology
  subject:
    topic: Biotechnologie
  subject:
    topic: Modèles biologiques
  subject:
    topic: Analyse de systèmes
  subject:
    topic: bioengineering
  subject:
    topic: systems analysis
  subject:
    topic: TECHNOLOGY & ENGINEERING
    topic: Biomedical
  subject:
    topic: MEDICAL
    topic: Family & General Practice
  subject:
    topic: MEDICAL
    topic: Allied Health Services
    topic: Medical Technology
  subject:
    topic: MEDICAL
    topic: Biotechnology
  subject:
    topic: MEDICAL
    topic: Lasers in Medicine
  subject:
    topic: Bioengineering
  classification: R856 .M47 2009eb
  classification: TA164 .M475 2009eb
  classification: 610.28
  classification: 2010 A-765
  classification: QT 36 M5915 2009
  related item:
    title info:
      title: Methods in bioengineering
    origin info:
      publisher: Boston [Mass.] : Artech House, ©2009
    identifier: (OCoLC)326517056
  related item:
    title info:
      title: Artech House methods in bioengineering series
  identifier: 9781596934078
  identifier: 1596934077
  identifier: 9781596934061
  identifier: 1596934069
  identifier: 2009279324
  identifier: http://proxy.library.tamu.edu/login
  record info:
    record content source: N$T
    record creation date: 100306
    record change date: 20230110223837.4
    record identifier: in00004637437
    language of cataloging:
      language term: eng