DNA and RNA Isolation Techniques for Non-Experts /
This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniq...
| Main Author: | |
|---|---|
| Corporate Author: | |
| Format: | eBook |
| Language: | English |
| Published: |
Cham :
Springer International Publishing : Imprint: Springer,
2022.
|
| Edition: | 1st ed. 2022. |
| Series: | Techniques in Life Science and Biomedicine for the Non-Expert,
|
| Subjects: | |
| Online Access: | Connect to the full text of this electronic book |
Table of Contents:
- 1-Basic Idea About Nucleic Acids For Non-Experts
- 2-General Steps During Isolation Of DNA And RNA
- 3-Phenol-Chloroform DNA Isolation Method
- 4-RNA Isolation By The Guanidinium-Acid-Phenol Method
- 5-Spin Column-Based Isolation Of Nucleic Acid
- 6-Isolation Of Plasmid DNA By Alkaline Lysis
- 7-DNA Isolation By Hydrophilic Ionic Liquid Treatment
- 8-Lithium Chloride Based Isolation Of DNA And RNA
- 9-Isolation Of Bacteriophage DNA By Peg Method
- 10-DNA Isolation By Chelex Method
- 11-DNA Isolation By Lysozyme And Proteinase K
- 12-Salting-Out Method For Blood Samples
- 13-CTAB Or SDA Based Isolation Of Plant's DNA
- 14-DNA Extraction By Spooling Method
- 15-Magnetic Bead-Based Nucleic Acid Isolation
- 16-Density Gradient-Based Nucleic Acid Isolation
- 17-DNA Extraction From Agarose Gel Though Paper-Strips
- 18-Transformation Or Genetic Modification Of Cells/Organisms
- 19-Gene Cloning And Vectors
- 20-Polymerase Chain Reaction (PCR)
- 21-Southern And Northern Blotting
- 22-Genome Mapping
- 23-Applications Of DNA Sequencing Technologies For Current Research.