Molecular biology techniques : a classroom laboratory manual /
| Main Authors: | , , , |
|---|---|
| Format: | eBook |
| Language: | English |
| Published: |
London, United Kingdom :
Elsevier/Academic Press,
2019.
|
| Edition: | Fourth edition. |
| Subjects: | |
| Online Access: | Connect to the full text of this electronic book |
Table of Contents:
- Getting oriented: practicing with micropipettes
- Purification and digestion of plasmid (vector) DNA
- Completion of vector preparation and polymerase chain reaction amplification of egfp
- Preparation on insert DNA (egfp) PCR product
- DNA ligation and transformation of Escherichia coli
- Screening of transformants, part I
- Screening of transformants, part II
- Analysis of DNA sequence from a positive clone
- Expressions of fusion protein from positive clones, SDS-PAGE and Western Blot: Part I
- Expressions of fusion protein from positive clones, SDS-PAGE and Western Blot: Part II
- Extraction of recombinant protein from Escherichia coli using a glutathione affinity culture
- Analysis of purification fractions
- Total RNA purification
- Analysis of gst:egfp mRNA levels by RT-qPCR: Part I
- Analysis of gst:egfp mRNA levels by RT-qPCR: Part II
- Analysis of gst:egfp mRNA levels by semiquantitative RT-PCR, Part I
- Analysis of gst:egfp mRNA levels by semiquantitative RT-PCR, Part II
- Culturing mammalian cells
- Transient transfection of mammalian cells
- RNAi-mediated knockdown of EGFP, Part I
- RNAi-mediated knockdown of EGFP, Part II
- RNAi-mediated knockdown of EGFP, Part III
- CRISPR-mediated knockout of EGFP, Part I
- CRISPR-mediated knockout of EGFP, Part II
- Advanced CRISPR, Part I
- Advanced CRISPR, Part II
- Interim laboratory session
- Advanced CRISPR, Part III
- Advanced CRISPR, Part IV
- Advanced CRISPR, Part V
- Advanced CRISPR, Part VI.