rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Molecular biology techniques
    sub title: a classroom laboratory manual
  name:
    name part: Carson, Sue (Susan)
    role:
      role term: creator
    role:
      role term: author.
  name:
    name part: Miller, Heather B.
    role:
      role term: author.
  name:
    name part: Srougi, Melissa C.
    role:
      role term: author.
  name:
    name part: Witherow, D. Scott
    role:
      role term: author.
  type of resource: text
  genre: bibliography
  genre: Laboratory Manual.
  origin info:
    place:
      place term: enk
    date issued: 2019
    edition: Fourth edition.
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource : illustrations
  table of contents: Getting oriented: practicing with micropipettes -- Purification and digestion of plasmid (vector) DNA -- Completion of vector preparation and polymerase chain reaction amplification of egfp -- Preparation on insert DNA (egfp) PCR product -- DNA ligation and transformation of Escherichia coli -- Screening of transformants, part I -- Screening of transformants, part II -- Analysis of DNA sequence from a positive clone -- Expressions of fusion protein from positive clones, SDS-PAGE and Western Blot: Part I -- Expressions of fusion protein from positive clones, SDS-PAGE and Western Blot: Part II -- Extraction of recombinant protein from Escherichia coli using a glutathione affinity culture -- Analysis of purification fractions -- Total RNA purification -- Analysis of gst:egfp mRNA levels by RT-qPCR: Part I -- Analysis of gst:egfp mRNA levels by RT-qPCR: Part II -- Analysis of gst:egfp mRNA levels by semiquantitative RT-PCR, Part I -- Analysis of gst:egfp mRNA levels by semiquantitative RT-PCR, Part II -- Culturing mammalian cells -- Transient transfection of mammalian cells -- RNAi-mediated knockdown of EGFP, Part I -- RNAi-mediated knockdown of EGFP, Part II -- RNAi-mediated knockdown of EGFP, Part III -- CRISPR-mediated knockout of EGFP, Part I -- CRISPR-mediated knockout of EGFP, Part II -- Advanced CRISPR, Part I -- Advanced CRISPR, Part II -- Interim laboratory session -- Advanced CRISPR, Part III -- Advanced CRISPR, Part IV -- Advanced CRISPR, Part V -- Advanced CRISPR, Part VI.
  note: Susan Carson, Heather B. Miller, D. Scott Witherow, Melissa C. Srougi.
  note: Includes bibliographical references.
  subject:
    topic: Molecular biology
    genre: Laboratory manuals
  subject:
    topic: Genetics
    topic: Technique
  subject:
    topic: Genetic Techniques
  subject:
    topic: Molecular Biology
    topic: methods
  subject:
    topic: CRISPR-Cas Systems
  classification: QU 25 C321m 2019eb
  identifier: 9780128157756
  identifier: 0128157755
  identifier: 9780128180242
  identifier: 0128180242
  identifier: 9780128157749
  identifier: 0128157747
  identifier: http://proxy.library.tamu.edu/login
  record info:
    record content source: OPELS
    record creation date: 190314
    record change date: 20230808172803.7
    record identifier: in00004535401
    language of cataloging:
      language term: eng