http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Getting oriented: practicing with micropipettes -- Purification and digestion of plasmid (vector) DNA -- Completion of vector preparation and polymerase chain reaction amplification of egfp -- Preparation on insert DNA (egfp) PCR product -- DNA ligation and transformation of Escherichia coli -- Screening of transformants, part I -- Screening of transformants, part II -- Analysis of DNA sequence from a positive clone -- Expressions of fusion protein from positive clones, SDS-PAGE and Western Blot: Part I -- Expressions of fusion protein from positive clones, SDS-PAGE and Western Blot: Part II -- Extraction of recombinant protein from Escherichia coli using a glutathione affinity culture -- Analysis of purification fractions -- Total RNA purification -- Analysis of gst:egfp mRNA levels by RT-qPCR: Part I -- Analysis of gst:egfp mRNA levels by RT-qPCR: Part II -- Analysis of gst:egfp mRNA levels by semiquantitative RT-PCR, Part I -- Analysis of gst:egfp mRNA levels by semiquantitative RT-PCR, Part II -- Culturing mammalian cells -- Transient transfection of mammalian cells -- RNAi-mediated knockdown of EGFP, Part I -- RNAi-mediated knockdown of EGFP, Part II -- RNAi-mediated knockdown of EGFP, Part III -- CRISPR-mediated knockout of EGFP, Part I -- CRISPR-mediated knockout of EGFP, Part II -- Advanced CRISPR, Part I -- Advanced CRISPR, Part II -- Interim laboratory session -- Advanced CRISPR, Part III -- Advanced CRISPR, Part IV -- Advanced CRISPR, Part V -- Advanced CRISPR, Part VI.
Susan Carson, Heather B. Miller, D. Scott Witherow, Melissa C. Srougi.