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Culture of animal cells : a manual of basic technique and specialized applications /

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Bibliographic Details
Main Author: Freshney, R. Ian
Format: eBook
Language:English
Published: Hoboken, N.J. : Wiley-Blackwell, [2010]
Edition:Sixth edition.
Subjects:
Tissue culture > Laboratory manuals.
Cell culture > Laboratory manuals.
Cells, Cultured.
Culture Techniques.
Tissue Culture Techniques > methods.
Laboratory Manuals.
Ebooks.
Electronic books.
Online Access:Connect to the full text of this electronic book
Table of Contents:
  • Frontmatter
  • Color Plates
  • Introduction
  • Biology of Cultured Cells
  • Laboratory Design, Layout, and Equipment
  • Equipment and Materials
  • Aseptic Technique
  • Safety, Bioethics, and Validation
  • Culture Vessels and Substrates
  • Defined Media and Supplements
  • Serum-Free Media
  • Preparation and Sterilization
  • Primary Culture
  • Subculture and Cell Lines
  • Cloning and Selection
  • Cell Separation
  • Characterization
  • Differentiation
  • Transformation and Immortalization
  • Contamination
  • Cryopreservation
  • Quantitation
  • Cytotoxicity
  • Specialized Cells
  • Stem Cells, Germ Cells, and Amniocytes
  • Culture of Tumor Cells
  • Three-Dimensional Culture
  • Scale-Up and Automation
  • Specialized Techniques
  • Training Programs
  • Problem Solving
  • In Conclusion
  • Appendix I: Calculations and Preparation of Reagents
  • Appendix II: Sources of Equipment and Materials
  • Appendix III: Suppliers and Other Resources
  • Appendix IV: Glossary
  • Appendix V: Cross-contaminated or Misidentified Cell Lines
  • Appendix VI: General Textbooks and Relevant Journals
  • References
  • Index.