Antibodies : a laboratory manual /

"The focus of Antibodies: A Laboratory Manual, 2nd Edition, will be unchanged from the original edition by Ed Harlow and David Lane and will cover both the production and use of antibodies in a way that is accessible to the nonimmunologist. The emphasis will be on contemporary, essential antibo...

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Bibliographic Details
Uniform Title:	Using antibodies.
Other Authors:	Greenfield, Edward A., 1957-
Format:	Book
Language:	English
Published:	Cold Spring Harbor, New York : Cold Spring Harbor Laboratory Press, ©2014.
Edition:	Second edition.
Subjects:	Immunoglobulins > Laboratory manuals. Immunochemistry > Laboratory manuals. Antibodies > isolation & purification. Immunochemistry. Laboratory Manuals.

Table of Contents:

Antibody production by the immune system
The antibody molecule
Antibody-antigen interactions
Antibody responses
Selecting the antigen
Immunizing animals
Generating monoclonal antibodies
Growing hybridomas
Characterizing antibodies
Antibody purification and storage
Engineering antibodies
Labeling antibodies
Immunoblotting
Immunoprecipiation
Immunoassays
Cell staining
Antibody screening using high throughput flow cytometry
Appendix I: Electrophoresis
Appendix II: Protein techniques
Appendix III: General information
Appendix IV: Bacterial expression
Appendix V: Cautions