Preparation of a soluble antigen from Theileria cervi and its application to various serologic tests : a thesis /
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| Format: | Thesis Book |
| Language: | English |
| Published: |
[College Station, Tex.] :
[Texas A&M University],
[1970]
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| Subjects: |
| Abstract: | ABSTRACT: Theileria cervi serologic antigen was obtained from the erythrocytes of a deer infected with T. cervi. The animal was bled by venipuncture, when 20 per cent of tis erythrocytes were parasitized. The blood was hemolyzed by freezing at -20°C. A second blood sample was hemolyzed by adding distilled water. The two antigens obtained were solubilized by sonication, titrated by complement-fixation (CF) reaction, pooled and used in CF, hemagglutination (HA) and precipitin tests. The solubility of the antigen in buffer solvents as determined by CF reaction was in direct proportion to the rise in pH. It was highest at pH 11. For the CF and HA tests however the pH 10 buffer was used as solvent. Thirty-four serums obtained from six deer infected with T. cervi were used in the CF and HA tests. Both tests detected the presence of antibody to the parasite. Two precipitin tests; the ring precipitin and the agar gel diffusion tests gave no valid results. |
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| Item Description: | Vita. "Major Subject: Veterinary Microbiology". "Submitted to the Graduate College of Texas A&M University in partial fulfillment of the requirements for the degree of Master of Science January 1970". |
| Physical Description: | vii, 48 leaves : tables ; 30 cm |
| Bibliography: | Includes bibliographical references. |