Handbook of ELISPOT : methods and protocols /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
New York :
Humana Press,
2018.
|
| Edition: | Third edition. |
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 1808. Springer protocols (Series) |
| Subjects: |
Table of Contents:
- Challenges in developing protein secretion assays at a single-cell level
- Mastering the computational challenges of Elispot plate evaluation
- Essential controls for ELISpot assay
- Automatic search of spots and color classification in ELISPOT assay
- Four color ImmunoSpot assays for identification of effector T-cell lineages
- Detection of cross-reactive B cells using the FluoroSpot assay
- Multiplex ImmunoSpot assays for the study of functional B cell subpopulations
- Detecting all immunoglobulin classes and subclasses in a multiplex 7 color ImmunoSpot assay
- Multiplexing T- and B-cell FLUOROSPOT assays : experimental validation of the multi-color ImmunoSpot software based on center of mass distance algorithm
- Multi-color FLUOROSPOT counting using ImmunoSpot Fluoro-X suite
- B-Cell ELISpot assay to quantify antigen-specific antibody-secreting cells in human peripheral blood mononuclear cells
- Identification of novel mycobacterial targets for murine CD4+ T-cells by IFNγ ELISPOT
- ELISPOT-based "Multi-Color FluoroSpot" to study type-specific and cross-reactive responses in memory B cells after Dengue and Zika virus infections
- Cultured ELISpot assay to investigate Dengue virus specific T-cell responses
- Ex vivo ELISpot assay to investigate Dengue virus specific T-cell responses
- Ex vivo ELISpot assay to investigate iNKT cell responses in acute Dengue infection
- Dendritic cell-based ELISpot assay for assessing T-cell IFN-γ responses in human peripheral blood mononuclear cells in Dengue envelope proteins
- Utilization of feline ELISpot to evaluate the immunogenicity of a T cell-based FIV MAP vaccine
- Detection and quantification of influenza A/H1N1 virus-speceifc memory B cells in human PBMCs using ELISpot assay
- Towards a full automation of the ELISpot assay for safe and parallelized immunomonitoring.