Mass spectrometry of proteins and peptides : methods and protocols.

Bibliographic Details
Other Authors: Lipton, Mary S., Paša-Tolić, Ljiljana
Format: Book
Language:English
Published: New York : Humana Press, 2009.
Edition:Second edition /
Series:Methods in molecular biology ; 492.
Subjects:
Table of Contents:
  • Generation and analysis of multidemensional protein identification technology datasets
  • Quantitative peptide and protein profiling by mass spectrometry
  • Label-free relative quantitation of prokaryotic proteomes using the accurate mass and time tag approach
  • Classical proteomics : two-dimensional electrophoresis/MALDI mass spectrometry
  • The use of difference in-gel electrophoresis for quantitation of protein expression
  • Liquid-chromatography-mass spectrometry of thylakoid membrane proteins
  • High accuracy mass spectrometry in large-scale analysis of protein phosphorylation
  • Manual validation of peptide sequence and sites of tyrosine phosphorylation from MS/MS spectra
  • Assigning glycosylation sites and microheterogeneities in glycoproteins by liquid chromatography/tandem mass spectrometry
  • Structure analysis of n-glycoproteins
  • Capillary zone electrophoresis-mass spectrometry for the characterization of isoforms of intact glycoproteins
  • Top-down proteomics of a high-field fourier transform ion cyclotron resonance mass spectrometer
  • Capillary isoelectric focusing/reversed phase liquid chromatography/mass spectrometry
  • Integrating accelerated tryptic digestion into proteomics workflows
  • Hydrogen/deuterium exchange mass spectrometry
  • Mass spectrometry detection and characterization of noncovalent protein complexes
  • Chemical cross-linking for protein-protein interaction studies
  • Tissue analysis with high-resolution imaging mass spectrometry
  • Proteomic global profiling for cancer biomarker discovery
  • Analysis of protein glycosylation and phosphorylation using liquid phase separation, protein microarray technology, and mass spectrometry
  • Transthyretin mass determination for detection of transthyretin familial amyloid
  • Characterization of microorganisms by MALDI mass spectrometry
  • Mass spectrometric characterization of neuropeptides
  • Peptide and protein ion/ion reactions in electrodynamic ion traps : tools and methods
  • Electron capture dissociation LC/MS/MS for bottom-up proteomics
  • Two-dimensional ion mobility analyses of proteins and peptides
  • Proteomics for validation of automated gene model predictions
  • Support vector machines for improved peptide identification from tandem mass spectrometry database search