Functional proteomics : methods and protocols /

Bibliographic Details
Other Authors: Thompson, Julie D., Schaeffer-Reiss, Christine, Ueffing, Marius
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [2008]
Series:Methods in molecular biology (Clifton, N.J.) ; 484.
Subjects:
Table of Contents:
  • A brief summary of the different types of mass spectrometers used in proteomics
  • Experimental setups and considerations to study microbial interactions
  • Plant proteomics
  • Methods for human CD8+ T lymphocyte proteome analysis
  • Label-free proteomics of serum
  • Flow cytometric analysis of cell membrane microparticles
  • Exosomes
  • Toward a full characterization of the human 20S proteasome subunits and their isoforms by a combination of proteomic approaches
  • Free-flow electrophoresis of the human urinary proteome
  • Versatile screening for binary protein-protein interactions by yeast two-hybrid mating
  • Native fractionation : isolation of native membrane-bound protein complexes from porcine rod outer segments using isopycnic density gradient centrifugation
  • Mapping the signaling pathways by functional interaction proteomics
  • Selection of recombinant antibodies by eukaryotic ribosome display
  • Production of protein arrays by cell-free systems
  • Nondenaturing mass spectrometry to study noncovalent protein/protein and protein/ligand complexes : technical aspects and application to the determination of binding stoichiometries
  • Protein processing characterized by a gel-free proteomics approach
  • Identification and characterization of N-glycosylated proteins using proteomics
  • Data standards and controlled vocabularies for proteomics
  • The PRIDE proteomics identifications database : data submission, query, and dataset comparison
  • Searching the protein interaction space through the MINT database
  • PepSeeker : mining information from proteomic data
  • Toward high-throughput and reliable peptide identification via MS/MS spectra
  • MassSorter : peptide mass fingerprinting data analysis
  • Database similarity searches
  • Protein multiple sequence alignment
  • Discovering biomedical knowledge from the literature
  • Protein subcellular localization prediction using artificial intelligence technology
  • Protein functional annotation by homology
  • Designability and disease
  • Prism : protein-protein interaction prediction by structural matching
  • Prediction of protein interaction based on similarity of phylogenetic trees
  • Large multiprotein structures modeling and simulation : the need for mesoscopic models
  • Dynamic pathway modeling of signal transduction networks : a domain-oriented approach