http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Plant-virus interactions -- Role of capsid proteins -- Role of plant virus movement proteins -- Multiple roles of viral replication proteins in plant RNA virus replication -- Role of silencing suppressor proteins -- Role of vector-transmission proteins -- Viral nucleic acid sequence/function analysis -- In vivo analyses of viral RNA translation -- In vitro analysis of translation enhancers -- Identification of plant virus IRES -- Analysis of geminivirus DNA replication -- Begomoviruses : molecular cloning and identification of replication origin -- Analysis of viroid replication -- Biochemical analyses of the interactions between viral polymerases and RNAs -- In situ detection of plant viruses and virus-specific products -- Detection of siRNAs and miRNAs -- Cloning of short interfering RNAs and virus-infected plants -- Solution structure probing of RNA structures -- RNA encapsidation assay -- Protein analysis and investigation of protein function -- Surface plasmon resonance anlaysis of interactions between replicase proteins of tomato bushy stunt virus -- Biochemical approaches for characterizing RNA-protein complexes in preparation for high resolution structure analysis -- Probing interactions between plant virus movement proteins and nucleic acids -- Movement profiles : a tool for quantitataive analysis of cell-to-cell movement of plant viral movement proteins -- Analysis of siRNA-suppressor of gene silencing interactions
Phosphorylation analysis of plant viral proteins -- Analysis of interactions between viral replicase proteins and plant intracellular membranes -- Membrane and protein dynamics in virus-infected plant cells -- Site-directed mutagenesis of whole viral genomes -- Viral proten-nucleic acid interaction : south (north)-western blot -- Protein-protein interactions : the yeast two-hybrid system -- NMR analysis of viral protein structures -- Localization of viral proteins in plant cells : protein tagging -- Microscopy/GFP/protein tagging/infections clones and other such tools -- Construction of infections cDNA clones for RNA viruses : TMV -- Construction of infectious cDNA clones for RNA viruses : turnip crinkle virus -- Construction of infectious clones for DNA viruses : masterviruses -- Construction of infectious clones of double-stranded DNA viruses of plant using citrus yellow mosaic virus as an example -- Insertion of introns : a strategy to facilitate assembly of infections full length clones -- Analysis of cell-to-cell and long-distance movement of apple latent spherical virus in infected plants using green, cyan, and yellow fluorescent proteins -- Agroinoculation : a simple procedure for systemic infection of plants with viruses -- Geminivirus : biolistic inoculation and molecular diagnosis -- Genomics, host factors and plant based studies -- Expression microarrays in plant-virus interaction -- Genome-wide screens for identification of host factors in viral replication -- Phosphorylation of movement proteins by the plasmodesmal-associated protein kinase -- Virus-induced gene silencing as a tool to identify host genes affecting viral pathogenicity -- Yeast two-hybrid assay to identify host-virus interactions
Previous edition has subtitle: from virus isolation to transgenic resistance.
Includes bibliographical references and index.
Methods in molecular biology (Clifton, N.J.) ; 451