Osteoporosis : methods and protocols /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana,
[2008]
|
| Series: | Methods in molecular biology ;
455. Methods in molecular biology (Clifton, N.J.) ; 455. |
| Subjects: |
Table of Contents:
- Isolation and culture of rodent osteoprogenitor cells
- Osteoclast culture and resorption assays
- Assessing new bone formation in neonatal calvarial organ cultures
- Detection of apoptosis of bone cells in vitro
- In vivo parathyroid hormone treatments and RNA isolation and analysis
- Assessment of bone formation assays : procedure and tissue analysis
- Ossicle and vossicle implant model systems
- Utility of the ovariectomized rat as a model for human osteoporosis in drug discovery
- Orchidectomy models of osteoporosis
- Gene delivery by adenoviruses
- Lentivirus delivery of shRNA constructs into osteoblasts
- Gene delivery by retrovirus
- Chromatin immunoprecipitation assays : application of ChIP-on-chip for definging dynamic transcriptional mechanisms in bone cells
- Identification of transcription factor target genes by ChIP display
- Application of the laser capture microdissection technique for molecular definition of skeletal cell differentiation in vivo
- Quantitative trait loci mapping
- In situ nuclear organization of regulatory machinery
- Bioluminescent imaging in bone
- Micro-computed tomography : a method for the non-destructive evaluation of the three-dimensional structure of biological specimens
- Fourier transform-infrared microspectroscopy and microscopic imaging
- Assessment of the in vivo adaptive response to mechanical loading
- Determination of bone's mechanical matrix properties by nanoindentation
- fluid flow assays.