Table of Contents:
  • The role of cell-free rabbit reticulocyte expression systems in functional proteomics
  • Advances in Insect-based cell-free protein expression
  • Advantages and applications of the batch-formatted E. coli cell-free expression system
  • Energetics in Escherichia coli-based batch cell-free systems
  • Disulfide bond formation in bacteria-based cell-free protein expression
  • The PURE system: a minimal cell-free translation system
  • Cell-free expression approaches for the production and characterization of membrane proteins
  • Cell-free expression for protein NMR
  • Cell-free synthesis of membrane proteins for X-ray crystallography
  • Bacterial cell-free expression systems for high-throughput protein production
  • Cell-free protein synthesis for protein microarrays
  • Cell-free protein expression screening and protein immobilization using protein microarrays
  • Cell-free protein expression labeling with fluorophores
  • Cell-free synthesis of defined protein conjugates by site-directed cotranslational labeling
  • C-terminal labeling of proteins using fluorescently conjugated puromycin derivatives
  • Translation engineering and synthetic biolog
  • Accelerated protein evolution using ribosome display
  • Application of in vitro virus (IVV) technique for high-throughput analysis of protein-protein interactions