In vitro transcription and translation protocols /

Bibliographic Details
Other Authors:	Grandi, Guido
Format:	Book
Language:	English
Published:	Totowa, N.J. : Humana Press, [2007]
Edition:	Second edition.
Series:	Methods in molecular biology (Clifton, N.J.) ; v. 375.
Subjects:	Molecular genetics > Laboratory manuals. Genetic transcription > Laboratory manuals. Genetic translation > Laboratory manuals. Molecular Biology. Protein Biosynthesis. Transcription, Genetic. Genetic Techniques. Molecular Biology > methods. Laboratory Manual.

Table of Contents:

Energy systems for ATP regeneration in cell-free protein synthesis reactions
Continuous-exchange protein-synthesizing systems
Cell-free production of integral membrane proteins on a preparative scale
SIMPLEX : Single-molecule PCR-linked in vitro expression : a novel method for high-throughput construction and screening of protein libraries
Methods for high-throughput materialization of genetic information based on the wheat germ cell-free expression system
Exogenous protein expression in Xenopus oocytes : basic procedures
In vitro translation to study HIV protease activity
The protein truncation test in mutation detection and molecular diagnosis
Creation of novel enantioselective lipases by SIMPLEX
In vitro transcription and translation coupled to two-dimensional electrophoresis for bacterial proteome analysis
In vitro screen of bioinformatically selected Bacillus anthracis vaccine candidates by coupled transcription, translation, and immunoprecipitation analysis
Functional expression of Type 1 rat GABA transporter in microinjected Xenopus laevis oocytes
Production of protein for nuclear magnetic resonance study using the wheat germ cell-free system.