http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Baculovirus and insect cell expression protocols
xiv, 470 pages : illustrations (some color) ; 24 cm.
Useful tips, widely used techniques, and quantifying cell metabolic behavior -- Introduction to baculovirus molecular biology -- Baculovirus transfer vectors -- Recombinant baculovirus isolation -- Gene expression in mammalian cells using MacMam, a modified baculovirus system -- Available lepidopteran insect cell line isolation from insect tissue -- Development of serum-free media for lepidopteran insect cell lines -- Routine maintenance and storage of lepidopteran insect cell lines and baculoviruses -- Small-scale protein production with the baculovirus expression vector system -- Recombinant protein production in large-scale agitated bioreactors using the baculovirus expression vector system -- Production of baculovirus-expressed recombinant proteins in wave bioreactors -- Protein production with recombinant baculoviruses in lepidopteran larvae -- Virus-like particle and viral vector production using the BEVS/insect cell system : adeno-associated virus-based products -- Transforming lepidopteran insect cells for continuous recombinant protein expression -- Transformation of Drosophila cell lines : an alternative approach to exogenous protein expression -- Transforming lepidopteran insect cells for improved protein processing -- Introduction to the use of baculovirsuses as biological insecticides -- Baculovirus insecticide production in insect larvae -- Evaluation of the insecticidal efficacy of wild type and recombinant baculoviruses -- Monitoring and visualization of baculovirus infection using green fluorescent protein strategy -- Alternative bioreactor strategy for probing infection and production -- Methods for gene silencing with RNAi -- Use of the baculovirus/insect cell system to study apoptosis -- Generation of envelope-modified baculoviruses for gene delivery into mammalian cells
Revised edition of: Baculovirus expression protocols. c1995.
Includes bibliographical references and index.
Methods in molecular biology (Clifton, N.J.) ; v. 388