Glycobiology protocols /

Bibliographic Details
Other Authors: Brockhausen, Inka, 1944-
Format: Book
Language:English
Published: Totowa, N.J. : Humana Press, [2006]
Series:Methods in molecular biology ; v. 347.
Subjects:
Table of Contents:
  • The application of fluorophore-assisted carbohydrate electrophoresis for the study of the dolichol pyrophosphate-linked oligosaccharides pathway in cell cultures and animal tissues
  • Partial purification of mannosylphosphorylundecaprenol synthase from micrococcus luteus : a useful enzyme for the biosynthesis of a variety of mannosylphosphorylpolyisoprenol products
  • Assay of dolichyl-phospho-mannose synthase reconstituted in a lipid matrix
  • O-mannosylation in mammalian cells
  • Methods for analysis of unusual forms of o-glycosylation
  • Structural analysis of naturally occurring sialic acids
  • Nonradioactive trans-sialidase screening assay
  • Structural determination of o-glycans by tandem mass spectrometry
  • Detailed structural analysis of n-glycans released from glycoproteins in SDS-page gel bands using HPLC combined with exoglycosidase array digestions
  • Molecular modeling of glycosyltransferases
  • Beta-Galactoside alpha2,6-sialyltransferase and the sialyl alpha2,6-galactosyl-linkage in tissues and cell lines
  • Visualizing intracellular distribution and activity of core 2 beta(1,6)n-acetylglucosaminyltransferase-I in living cells
  • Gene expression analysis of glycosylation-related genes by real-time polymerase chain reaction
  • Analysis of the glycodynamics of primary osteoblasts and bone cancer cells
  • Micromethods for the characterization of lipid a core and o-antigen lipopolysaccharide
  • Assays for a galactosyltransferase involved in the assembly of the O7-antigen repeat unit of escherichia coli
  • High-throughput quantitation of metabolically labeled anionic glycoconjugates by scintillation proximity assay utilizing binding to cationic dyes
  • Quantitative analysis of mucins in mucosal secretions using indirect enzyme-linked immunosorbent assay
  • Molecular dissection of the mouse zona pellucida : an electron microscopic perspective utilizing high-resolution colloidal-gold labeling methods
  • Soluble adamantly glycosphingolipid analogs as probes of glycosphingolipid function
  • Diagnosis of Krabbe disease by use of a natural substrate
  • In vitro assays of the functions of calnexin and calreticulin, lectin-chaperones of the endo-plasmic reticulum
  • Quantitative measurement of selectin-ligand interactions : assays to identify a sweet pill in a library of carbohydrates
  • Binding and inhibition assays for siglecs