rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    non sort: The
    title: role of tumor necrosis factor-alpha (TNF-[alpha]) in host resistance to experimental pulmonary tuberculosis
    sub title: a dissertation
  title info:
    title: Role of tumor necrosis factor-alpha (TNF-α) in host resistance to experimental pulmonary tuberculosis
  name:
    name part: Lasco, Todd Michael.
    role:
      role term: creator
  type of resource: text
  genre: abstract or summary
  origin info:
    place:
      place term: txu
    date issued: 2002
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xii, 130 leaves : illustrations ; 28 cm.
  abstract: ABSTRACT: The purpose of this study was to examine the role of TNF-[alpha] in host immunity using an experimental model of pulmonary tuberculosis. In this study, we focused on three leukocyte-rich guinea pig cell populations, bronchoalveolar lavage (BAL) cells, resident peritoneal cells (PC), and splenocytes (SPC). Each cell population was stimulated with either live attenuated M. tuberculosis H37Ra, or live/heat-killed virulent M. tuberculosis H37Rv (MOI 1:100). Each cell population was determined to proliferate in response to heat-killed virulent H37Rv, whereas no measurable proliferative response could be detected upon stimulation with live mycobacteria. Additionally, this proliferative capacity was enhanced significantly upon prior vaccination with M. bovis BCG in the case of SPC and PC populations. Accordingly, in a parallel set of experiments we found a strong positive correlation between bioactive TNF-[alpha] production and prior vaccination with BCG. Although, this same correlation was not detected at the mRNA level, we determined this to be purely antigen-specific process, whereby a non-specific stimulus, lipopolysaccharide (LPS), failed to induce this effect on BAL-cells, SPC, and PC populations stimulated with M. tuberculosis PPD, we determined that upon prior aerosol challenge with M. tuberculosis H37Rv, the opposite was true. That is, BAL-cells, SPC an PC obtained from non-vaccinated counterparts. Furthermore, both recombinant guinea pig TNF-[alpha] (rgpTNF-[alpha]) and polyclonal rabbit anti- rgpTNF-[alpha] reagents were developed and utilized to modulate TNF-[alpha] availability in a series of in vitro and in vivo experiments. We determined that the in vitro effect of TNF-[alpha] on PPD-induced T-cell proliferation is highly dependent on the concentration of TNF-[alpha]. While PPD-induced proliferation from SPC correlated with persistent levels of TNF-[alpha] in the supernatant, further addition of TNF-[alpha] (with rgpTNF-[alpha]) suppressed this pro…
  note: by Todd Michael Lasco.
  note: Vita.
  note: "Major Subject: Medical Sciences".
  note: "Submitted to the Office of Graduate Studies Texas A&M University in partial fulfillment for the requirements for the degree of Doctor of Philosophy December 2002."
  note: Approved as to style and content by: David N. McMurrary, Roger Smith III, James E. Samuel, Vernon L. Tesh, John M. Quarles.
  note: Ph. D. Texas A&M University System Health Science Center 2002.
  note: Includes bibliographical references (leaves 117-129).
  subject:
    topic: Tuberculosis, Pulmonary
    topic: therapy
  subject:
    topic: Tumor Necrosis Factor-alpha
    topic: therapeutic use
  subject:
    topic: Major Medical Sciences
  classification: W 4 AT4 L342r 2002
  record info:
    record content source: TMV
    record creation date: 060911
    record change date: 20191203131536.0
    record identifier: in00004503185