rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    non sort: The
    title: role of TNF-α in the resistance of guinea pigs to Mycobacterium tuberculosis infection
    sub title: a dissertation
  name:
    name part: Cho, Hyosun.
    role:
      role term: creator
  type of resource: text
  genre: abstract or summary
  origin info:
    place:
      place term: txu
    date issued: 2005
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xi, 104 leaves : illustrations ; 28 cm.
  abstract: ABSTRACT: TNF-α plays an important role in the host immune response to infection with the intracellular pathogen Mycobacterium tuberculosis. It is essential for the formation of protective tuberculous granulomas and regulates the expression of other cytokines which contribute to a protective immune response. In this study, using recombinant guinea pig (rgp) TNF-α or neutralizing polyclonal anti-rgpTNF-α antibody, we have investigated the role of guinea pig TNF-α in guinea pig macrophage cultures as well as cocultures of immune lymphocytes and macrophages infected with M. tuberculosis. Resident alveolar and peritoneal macrophages were isolated from guinea pig and stimulated with rgpTNF-α or infected with either the attentuated H37Ra or virulent H37Rv strains of M. tuberculosis in the presence of rgpTNF-α. IL-12 p40 mRNA was up-regulated in a dose-dependent manner by rgp TNF-α alone. In infected macrophages, a lower dose of rgpTNF-α intensified the mRNA levels of TNF-α and IL-12 p40. However, higher doses of rgpTNF-α suppressed TNF-α and IL-12 p40 mRNA, which suggests that the dose of TNF-α is crucial to the stimulation of optimal expression of protective cytokines. Infected resident alveolar and peritoneal macrophages treated with anti-gpTNF-α antibody to block endogenous TNF-α activity exhibited increased intracellular mycobacterial growth. TNF-α is known to contribute to the activation of innate immunity and the transition to antigen-specific adaptive immunity in tuberculosis. Therefore, we have examined the effect of TNF-α on lymphocyte activation in unvaccinated and BCG-vaccinated guinea pigs. Splenocytes were stimulated with PPD or ConA in the presence or absence of rgpTNF-α for 96 hours. rgpTNF-α alone was able to stimulate a significant degree of proliferation in splenocytes. The addition of rgpTNF-α enhanced the proliferation of PPD-stimulated splenocytes from BCG-vaccinated guinea pigs, in conjunction with upregulation of the levels of Type 1 cytokine mRNA (…
  note: by Hyosun Cho.
  note: Vita.
  note: "Major Subject: Medical Sciences".
  note: "Submitted to the Graduate School of Biomedical Sciences of The Texas A&M University System Health Science Center in partial fulfillment for the requirements for the degree of Doctor of Philosophy December 2005."
  note: Approved as to style and content by: David N. McMurrary, Roger Smith III, James E. Samuel, Vernon L Tesh, John M. Quarles.
  note: Ph. D. Texas A&M University System Health Science Center 2005.
  note: Includes bibliographical references (leaves 87-103).
  subject:
    topic: Tumor Necrosis Factor-alpha
    topic: physiology
  subject:
    topic: Tumor Necrosis Factor-alpha
    topic: therapeutic use
  subject:
    topic: Mycobacterium tuberculosis
  subject:
    topic: Major Medical Sciences
  classification: W 4 AT4 C5451r 2005
  record info:
    record content source: TMV
    record creation date: 060912
    record change date: 20191203131538.0
    record identifier: in00004502595