Cardiac gene expression : methods and protocols /

Bibliographic Details
Other Authors:	Zhang, Jun, 1968-, Rokosh, Gregg
Format:	Book
Language:	English
Published:	Totowa, N.J. : Humana Press, [2007]
Series:	Methods in molecular biology (Clifton, N.J.) ; v. 366.
Subjects:	Myocardium > Laboratory manuals. Gene expression > Laboratory manuals. Genetic regulation > Laboratory manuals. Gene Expression Regulation. Gene Expression Profiling. Myocardium. Laboratory Manual.

Table of Contents:

Microarray analysis of gene expression in murine cardiac graft infiltrating cells
Expression profiling using Affymetrix GeneChip® probe arrays
Serial analysis of gene expression (SAGE) : a useful tool to analyze the cardiac transcriptome
Functional genomics by cDNA subtractive hybridization
Statistical methods in cardiac gene expression profiling : from image to function
Measurement of cardiac gene expression by reverse transcription polymerase chain reaction (RT-PCR)
Quantitative (real time) RT-PCR in cardiovascular research
RNase protection assay for quantifying gene expression levels
In situ hybridization : a technique to study localization of cardiac gene expression
Characterization of cis-regulatory elements and transcription factor binding : gel mobility shift assay
Mapping transcriptional start sites and in silico DNA footprinting
Characterization of cardiac gene promoter activity : reporter constructs and heterologous promoter studies
Comparitive genomics : a tool to functionally annotate human DNA
Developing computational resources in cardiac gene expression
In silico analysis of SNPs and other high-throughput data
Discovery and identification of sequence polymorphisms and mutations with MALDI-TOF MS
Conditional targeting : inducible deletion by Cre recombinase
Cardiomyocyte preparation, culture, and gene transfer
Adeno-associated viral vector-delivered hypoxia-inducible gene expression in ischemic hearts
Lentivirus-mediated gene expression