High content screening : a powerful approach to systems cell biology and drug discovery /

Bibliographic Details
Other Authors:	Taylor, D. Lansing, Haskins, Jeffrey R., Giuliano, Kenneth A.
Format:	Book
Language:	English
Published:	Totowa, N.J. : Humana Press, [2007]
Series:	Methods in molecular biology (Clifton, N.J.) ; v. 356.
Subjects:	Biological systems > Research > Methodology. Computational biology. Drug development > Computer simulation. Systems Biology > methods. Drug Design. Image Processing, Computer-Assisted > methods.

Table of Contents:

Past, present, and future of high content screening and the field of cellomics
A pharmaceutical company user's perspective on the potential of high content screening in drug discovery
Linking microscopy and high content screening in large-scale biomedical research
Requirements, features, and performance of high content screening platforms
Characteristics and value of directed algorithms in high content screening
Characteristics and value of machine learning for imaging in high content screening
Tools for quantitative and validated measurements of cells
Automated cell plating and sample treatments for fixed cells in high content assays
Differentiating primary human cells in rapid-throughput discovery applications
Use of the CellCard system for analyzing multiple cell types in parallel
Reagents to measure and manipulate cell functions
Fluorescent proteins and engineered cell lines
Optimizing the integration of immunoreagents and fluorescent probes for multiplexed high content screening assays
The HaloTag : a novel technology for cell imaging and protein analysis
Protein labeling with FlAsH and ReAsH
Exploiting network biology to improve drug discovery
Physiological indicators of cell function
The use of siRNA to validate immunofluorescence studies
Caged substrates applied to high content screening : an introduction with an eye to the future
Overview of informatics for high content screening
Large-scale data management for high content screening
An integrated biomedical knowledge extraction and analysis platform : using federated search and document clustering technology
Visualization of high content screening data
Pathway mapping tools for analysis of high content data
Systems biology in cancer research : genomics to cellomics
Target validation in drug discovery
High content screening as a screening tool in drug discovery
Discovery of protein kinase phosphatase inhibitors
High content translocation assays for pathway profiling
In vitro cytotoxicity assessment
Neurite outgrowth in retinal ganglion cell culture