Fluorescent energy transfer nucleic acid probes : designs and protocols /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2006]
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| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 335. |
| Subjects: |
Table of Contents:
- Selection of fluorophore and quencher pairs for fluorescent nucleic acid hybridization probes
- Choosing reporter-quencher pairs for efficient quenching through formation of intramolecular dimers
- Detection of DNA hybridization using induced fluorescence resonance energy transfer
- Detecting RNA/DNA hybridization using double-labeled donor probes with enhanced fluorescence resonance energy transfer signals
- Oscillating probe for dual detection of 5'PO₄ and 5'OH DNA breaks in tissue sections
- Using molecular beacons for sensitive fluorescence assays of the enzymatic cleavage of nucleic acids
- A continuous assay for DNA cleavage using molecular break lights
- Homogenous detection of nucleic acids using self-quenched polymerase chain reaction primers labeled with a single fluorophore (LUXTM Primers)
- Use of self-quenched, fluorogenic LUXTM primers for gene expression profiling
- TaqMan® reverse transcriptase-polymerase chain reaction coupled with capillary electrophoresis for quantification and identification of bcr-abl transcript type
- Quantitative TaqMan® assay for the detection and monitoring of cytomegalovirus infection in organ transplant patients
- Real-time detection and quantification of telomerase activity utilizing energy transfer primers
- Invader® assay for single-nucleotide polymorphism genotyping and gene copy number evaluation
- Real-time quantitative polymerase chain reaction analysis of mitochondrial DNA point mutation
- Multiplex single-nucleotide polymorphism detection by combinatorial fluroscence energy transfer tags and molecular affinity
- High-throughput genotyping with energy transfer-labeled primers
- Distance determination in protein-DNA complexes using fluorescence resonance energy transfer
- Multi-flurophore fluorescence resonance energy transfer for probing nucleic acids structure and folding
- Fluorescent DNAzyme biosensors for metal ions based on catalytic molecular beacons
- Fluroscent energy transfer readout of an aptazyme-based biosensor
- Fluorescence resonance energy transfer in the studies of guanine quadruplexes
- Solution-phase molecular-scale computation with deoxyribozyme-based logic gates and fluorescent readouts