http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Fluorescent energy transfer nucleic acid probes
xv, 372 pages : illustrations ; 24 cm. + 1 CD-ROM (4 3/4 in.).
Selection of fluorophore and quencher pairs for fluorescent nucleic acid hybridization probes -- Choosing reporter-quencher pairs for efficient quenching through formation of intramolecular dimers -- Detection of DNA hybridization using induced fluorescence resonance energy transfer -- Detecting RNA/DNA hybridization using double-labeled donor probes with enhanced fluorescence resonance energy transfer signals -- Oscillating probe for dual detection of 5'PO₄ and 5'OH DNA breaks in tissue sections -- Using molecular beacons for sensitive fluorescence assays of the enzymatic cleavage of nucleic acids -- A continuous assay for DNA cleavage using molecular break lights -- Homogenous detection of nucleic acids using self-quenched polymerase chain reaction primers labeled with a single fluorophore (LUXTM Primers) -- Use of self-quenched, fluorogenic LUXTM primers for gene expression profiling -- TaqMan® reverse transcriptase-polymerase chain reaction coupled with capillary electrophoresis for quantification and identification of bcr-abl transcript type -- Quantitative TaqMan® assay for the detection and monitoring of cytomegalovirus infection in organ transplant patients -- Real-time detection and quantification of telomerase activity utilizing energy transfer primers -- Invader® assay for single-nucleotide polymorphism genotyping and gene copy number evaluation -- Real-time quantitative polymerase chain reaction analysis of mitochondrial DNA point mutation -- Multiplex single-nucleotide polymorphism detection by combinatorial fluroscence energy transfer tags and molecular affinity -- High-throughput genotyping with energy transfer-labeled primers -- Distance determination in protein-DNA complexes using fluorescence resonance energy transfer -- Multi-flurophore fluorescence resonance energy transfer for probing nucleic acids structure and folding -- Fluorescent DNAzyme biosensors for metal ions based on catalytic molecular beacons -- Fluroscent energy transfer readout of an aptazyme-based biosensor -- Fluorescence resonance energy transfer in the studies of guanine quadruplexes -- Solution-phase molecular-scale computation with deoxyribozyme-based logic gates and fluorescent readouts
Includes bibliographical references and index.
Methods in molecular biology (Clifton, N.J.) ; v. 335