Breast cancer research protocols /
| Other Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2006]
|
| Series: | Methods in molecular medicine.
120. |
| Subjects: |
Table of Contents:
- Breast tissue banking : collection, handling, storage, and release of tissue for breast cancer research
- Frozen biopsy collection and storage
- Serial biopsies/FNAs and their assessment
- Breast tissue microarrays
- Preparation of tumor homogenates for subsequent preparation of cytosols, membrane fractions, RNA, and DNA
- Isolation of RNA from tumor samples : single-step guanidinium acid-phenol method
- Isolation of DNA from tumor samples
- Laser-assisted microdissection and isolation of DNA and RNA
- Traditional and established indicators of prognosis and treatment success
- Sentinel lymph node biopsy in breast cancer
- Pathological evaluation of axillary sentinel lymph nodes in breast cancer
- Measurement of estrogen receptor status by immunocytochemistry in paraffin wax sections
- Markers of apoptosis
- Quantitative angiogenesis in breast cancer
- Immunohistochemistry
- Detection of aberrant glycosylation in breast cancer using lectin histochemistry
- SDS-PAGE and western blotting to detect proteins and glycoproteins of interest in breast cancer research
- Breast cancer proteomics using two-dimensional electrophoresis
- Procedures for the quantitative protein determination of urokinase and its inhibitor PAI-1 in human breast cancer tissue extracts using ELISA
- Fluorescence in situ hybridization and comparative genomic hybridization
- Fluorescence in situ hybridization assessment of c-myc amplification in breast cancer tissues
- Detection of HER2 gene amplification by FISH in breast cancer
- In situ hybridization combined with immunohistochemistry to localize gene expression
- Quantitation of RNA by ribonuclease protection assay
- Identification of steroid hormone-regulated genes in breast cancer
- Sequencing of the tumor suppressor gene TP 53
- Expression profiling using cDNA microarrays
- Gene expression analysis using filter cDNA microarrays
- Methods to analyze the effects of the urokinase system on cancer cell adhesion, proliferation, migration, and signal transduction events
- Phosphospecific antibodies as a tool to study in vivo regulation of BRCA1 after DNA damage
- Models of hormone resistance in vitro and in vivo
- Generation of genetically modified embryonic stem cells for the development of knockout mouse animal model systems
- In vivo xenograft models of breast cancer metastatsis
- Neoadjuvant endocrine therapy models
- Primary mouse endothelial cell culture for assays of angiogenesis