Placenta and trophoblast : methods and protocols /

Bibliographic Details
Other Authors:	Soares, Michael J., Hunt, Joan S.
Format:	Book
Language:	English
Published:	Totowa, N.J. : Humana Press, [2006]
Series:	Methods in molecular medicine. 121-122.
Subjects:	Placenta. Molecular biology. Molecular Biology.

Table of Contents:

v. 1
Placenta and trophoblast : methods and protocols : overview I
Methodologies to study implantation in mice
Blastocyst culture
Isolation of hormone responsive uterine stromal cells : an in vitro model for stromal cell proliferation and differentiation
Rat decidual cell cultures
The immortalization of human endometrial cells
Sheep uterine gland knockout (UGKO) model
A baboon model for inducing endometriosis
A baboon model for simulating pregnancy
The common marmoset monkey as a model for implantation and early pregnancy research
Mouse trophoblast stem cells
Connexins and trophoblast cell lineage development
Rcho-1 trophoblast stem cells : a model system for studying trophoblast cell differentiation
Bovine trophoblast cell culture systems : a technique to culture bovine trophoblast cells without feeder cells
In vitro induction of trophoblast from human embryonic stem cells
Isolation and culture of term human trophoblast cells
Production of human trophoblast cell lines
Culture and transfection of human choriocarcinoma cells
In vitro methods for studying vascularization of the murine allantois and allantoic union with the chorion
Phenotypic analysis of the mouse placenta
Phenotypic analysis of the rat placenta
Analysis of the structure of the ruminant placenta : methods of fixation, embedding and antibody localization at light and electron microscope levels
Characterization of the bovine placenta by cytoskeleton, integrin receptors and extracellular matrix
Molecular markers for human placental investigation
Correlative microscopy of ultrathin cryosections in placental research
Vascular corrosion casting of the uteroplacental and fetoplacental vasculature in mice
Analysis of fetal and maternal microvasculature in ruminant placentomes by corrosion casting
Microarray analysis of trophoblast cells
Gene expression microarray data analysis of decidual and placental cell differentiation
Assays to determine allelic usage of gene expression in the placenta
Adenoviral-mediated gene delivery to trophoblast cells
v. 2
Placenta and trophoblast : methods and protocols : overview II
In vivo analysis of trophoblast cell invasion in the human
In vitro analysis of trophoblast invasion
An in vitro model of trophoblast invasion of spiral arteries
In vivo models for studying homing and function of the murine uterine natural killer cells
Immune and trophoblast cells at the Rhesus monkey maternal-fetus interface
Methods for isolation of cells from the human fetal-maternal interface
In vitro models for studying human uterine and placental marcophages
Macrophage-trophoblast interactions
Methods for evaluating histocompatibility antigen gene expression in the baboon
Analysis of the soluble isoforms of HLA-G : mRNAs and proteins
In vivo techniques for studying placental nutrient uptake, metabolism, and transport
In vitro models for studying trophoblast transcellular transport
In vitro methods for studying human placental amino acid transport : placental plasma membrane vesicles
In vitro methods for studying human placental amino acid transport
placental villous fragments
Methods for investigating placental fatty acid transport
Heterologous expression systems for studying placental transporters
Analysis of trophoblast giant cell steroidogenesis in primary cultures
Establishment of an ELISA for the detection of native bovine pregnancy-associated glycoproteins secreted by trophoblast binucleate cells
Alkaline phosphatase fusion proteins as tags for identifying targets for placental ligands
Bacterial expression of prolactin family proteins
Analysis of placental regulation of hematopoiesis
Methods for studying interferon tau stimulated genes
Reduced uterine perfusion pressure (RUPP) model for studying cardiovascular-renal dysfunction in response to placental ischemia
In vivo rat model of preeclampsia
A novel mouse model for preeclampsia by transferring activated Th1 bells into normal pregnant mice
Working with oxygen and oxidative stress in vitro
Hypobaric hypoxia as a tool to study pregnancy-dependent responses at the maternal-fetal interface
Infection with Listeria monocytogenes as a probe for placental immunological function